Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3256060..3256714 Replicon chromosome
Accession NZ_CP024683
Organism Citrobacter freundii strain UMH13

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag CUC45_RS15855 Protein ID WP_003026936.1
Coordinates 3256060..3256467 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag CUC45_RS15860 Protein ID WP_003026938.1
Coordinates 3256448..3256714 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC45_RS15835 3252008..3253741 - 1734 WP_032950023.1 single-stranded-DNA-specific exonuclease RecJ -
CUC45_RS15840 3253747..3254460 - 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CUC45_RS15845 3254484..3255380 - 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
CUC45_RS15850 3255494..3256015 + 522 WP_003026933.1 flavodoxin FldB -
CUC45_RS15855 3256060..3256467 - 408 WP_003026936.1 protein YgfX Toxin
CUC45_RS15860 3256448..3256714 - 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
CUC45_RS15870 3256971..3257951 + 981 WP_003026944.1 tRNA-modifying protein YgfZ -
CUC45_RS15875 3258045..3258704 - 660 WP_003026947.1 hemolysin III family protein -
CUC45_RS15880 3258867..3259178 - 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
CUC45_RS15885 3259231..3259959 + 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
CUC45_RS15890 3260080..3261513 + 1434 WP_032950025.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T88344 WP_003026936.1 NZ_CP024683:c3256467-3256060 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T88344 NZ_CP024683:c3256467-3256060 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGCCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT88344 WP_003026938.1 NZ_CP024683:c3256714-3256448 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88344 NZ_CP024683:c3256714-3256448 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References