Detailed information of TA system TA088332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 483036..483656
• Replicon: chromosome
• Accession: NZ_CP024683
• Organism: Citrobacter freundii strain UMH13

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: CUC45_RS02210
• Protein ID: WP_002892050.1
• Coordinates: 483036..483254 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CUC45_RS02215
• Protein ID: WP_032948706.1
• Coordinates: 483282..483656 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC45_RS02175	478394..479023	+	630	WP_032948704.1	membrane protein	-
CUC45_RS02180	479088..479441	+	354	WP_032948705.1	DUF1428 family protein	-
CUC45_RS02185	479493..481046	-	1554	WP_003835927.1	EAL domain-containing protein	-
CUC45_RS02190	481235..481495	+	261	WP_003021719.1	type B 50S ribosomal protein L31	-
CUC45_RS02195	481497..481637	+	141	WP_005125190.1	type B 50S ribosomal protein L36	-
CUC45_RS02200	481716..482186	-	471	WP_003021724.1	YlaC family protein	-
CUC45_RS02205	482303..482854	-	552	WP_003021728.1	maltose O-acetyltransferase	-
CUC45_RS02210	483036..483254	-	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
CUC45_RS02215	483282..483656	-	375	WP_032948706.1	Hha toxicity modulator TomB	Antitoxin
CUC45_RS02220	484144..487293	-	3150	WP_003021736.1	multidrug efflux RND transporter permease subunit	-
CUC45_RS02225	487316..488509	-	1194	WP_003835922.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T88332 WP_002892050.1 NZ_CP024683:c483254-483036 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T88332 NZ_CP024683:c483254-483036 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14408.18 Da
• Isoelectric Point: 5.5154

>AT88332 WP_032948706.1 NZ_CP024683:c483656-483282 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFHCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT88332 NZ_CP024683:c483656-483282 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCACTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2