Detailed information of TA system TA088331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3977..4620
• Replicon: plasmid pUMH14_1
• Accession: NZ_CP024681
• Organism: Citrobacter freundii strain UMH14

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CUC46_RS23750
• Protein ID: WP_121572286.1
• Coordinates: 4204..4620 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CUC46_RS23745
• Protein ID: WP_121572285.1
• Coordinates: 3977..4207 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC46_RS23720	358..720	-	363	WP_121572281.1	hypothetical protein	-
CUC46_RS23725	809..1120	-	312	WP_121572282.1	hypothetical protein	-
CUC46_RS23735	1692..2630	+	939	WP_121572283.1	hypothetical protein	-
CUC46_RS23740	2678..3703	-	1026	WP_121572284.1	hypothetical protein	-
CUC46_RS23745	3977..4207	+	231	WP_121572285.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CUC46_RS23750	4204..4620	+	417	WP_121572286.1	type II toxin-antitoxin system VapC family toxin	Toxin
CUC46_RS23755	4766..5836	-	1071	WP_141227224.1	hypothetical protein	-
CUC46_RS23760	6627..7256	-	630	WP_063136851.1	DUF1349 domain-containing protein	-
CUC46_RS23765	7338..8519	-	1182	WP_121572288.1	MFS transporter	-
CUC46_RS23770	8553..9311	-	759	WP_047732337.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..134386	134386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15133.65 Da
• Isoelectric Point: 6.2225

>T88331 WP_121572286.1 NZ_CP024681:4204-4620 [Citrobacter freundii]
VNKTYMLDTCICSFIMREQPEAVLKHLEQAVLRNQRIVIPVITYAEMRFGATGPKVSPRHIELVDAFCARLDAILPWDSA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGVVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T88331 NZ_CP024681:4204-4620 [Citrobacter freundii]
GTGAACAAAACCTATATGCTGGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCATCT
GGAGCAGGCGGTGCTGCGCAACCAGCGCATCGTTATCCCGGTCATCACCTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGTCTCGCCGCGCCACATTGAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACAGCGCC
GCGGTGGACGCCACCACGGAGATTAAGGTCGCGCTGCGGCTCGCCGGAACCCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCACGCCATTGCGGCCGGCGTCGTGCTGGTGACGAATAATGTGCGGGAGTTTGAGCGGGTGCCGGGCCTGGTACTGG
AAGACTGGGTTAAATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8779.86 Da
• Isoelectric Point: 4.1211

>AT88331 WP_121572285.1 NZ_CP024681:3977-4207 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVPPTWDSFLELEKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT88331 NZ_CP024681:3977-4207 [Citrobacter freundii]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCCGCCGACCTGGGATTCATTCCTGGAGCTCG
AAAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCACCGACGAAGGACGATTTGACCTGTGA