Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4690933..4691449 Replicon chromosome
Accession NZ_CP024680
Organism Citrobacter freundii strain UMH14

Toxin (Protein)


Gene name relE Uniprot ID A0A0D7LMW6
Locus tag CUC46_RS22905 Protein ID WP_003839578.1
Coordinates 4691165..4691449 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0J1MV10
Locus tag CUC46_RS22900 Protein ID WP_003839576.1
Coordinates 4690933..4691175 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC46_RS22880 4685948..4687081 + 1134 WP_121572199.1 amidohydrolase/deacetylase family metallohydrolase -
CUC46_RS22885 4687065..4688183 + 1119 WP_048219848.1 DgaE family pyridoxal phosphate-dependent ammonia lyase -
CUC46_RS22890 4688180..4688920 + 741 WP_003025772.1 KDGP aldolase family protein -
CUC46_RS22895 4688942..4690855 + 1914 WP_003839574.1 BglG family transcription antiterminator -
CUC46_RS22900 4690933..4691175 + 243 WP_003839576.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CUC46_RS22905 4691165..4691449 + 285 WP_003839578.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CUC46_RS22910 4691453..4691917 - 465 WP_003839580.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
CUC46_RS22915 4692024..4694162 - 2139 WP_003844922.1 anaerobic ribonucleoside-triphosphate reductase -
CUC46_RS22920 4694540..4695124 - 585 WP_003839584.1 fructose PTS transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10866.68 Da        Isoelectric Point: 10.0482

>T88330 WP_003839578.1 NZ_CP024680:4691165-4691449 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
REHSAVYLDANKRL

Download         Length: 285 bp

>T88330 NZ_CP024680:4691165-4691449 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAA
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.34 Da        Isoelectric Point: 5.0651

>AT88330 WP_003839576.1 NZ_CP024680:4690933-4691175 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Download         Length: 243 bp

>AT88330 NZ_CP024680:4690933-4691175 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7LMW6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MV10

References