Detailed information of TA system TA088319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1752488..1753127
• Replicon: chromosome
• Accession: NZ_CP024680
• Organism: Citrobacter freundii strain UMH14

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: CUC46_RS08565
• Protein ID: WP_003020221.1
• Coordinates: 1752951..1753127 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CUC46_RS08560
• Protein ID: WP_123924900.1
• Coordinates: 1752488..1752904 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC46_RS08540	1747534..1748478	-	945	WP_003843727.1	ABC transporter permease	-
CUC46_RS08545	1748479..1749495	-	1017	WP_121571759.1	ABC transporter ATP-binding protein	-
CUC46_RS08550	1749512..1750657	-	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
CUC46_RS08555	1750986..1752395	-	1410	WP_044702348.1	PLP-dependent aminotransferase family protein	-
CUC46_RS08560	1752488..1752904	-	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUC46_RS08565	1752951..1753127	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUC46_RS08570	1753391..1754794	+	1404	WP_121571760.1	cytochrome ubiquinol oxidase subunit I	-
CUC46_RS08575	1754794..1755804	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
CUC46_RS08580	1755804..1755929	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
CUC46_RS08585	1756119..1756349	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T88319 WP_003020221.1 NZ_CP024680:c1753127-1752951 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T88319 NZ_CP024680:c1753127-1752951 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT88319 WP_123924900.1 NZ_CP024680:c1752904-1752488 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT88319 NZ_CP024680:c1752904-1752488 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCAGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36