Detailed information of TA system TA088317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5131978..5132494
• Replicon: chromosome
• Accession: NZ_CP024679
• Organism: Citrobacter freundii strain UMH15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0D7LMW6
• Locus tag: CUC47_RS25595
• Protein ID: WP_003839578.1
• Coordinates: 5132210..5132494 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CUC47_RS25590
• Protein ID: WP_061548940.1
• Coordinates: 5131978..5132220 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC47_RS25570	5126993..5128126	+	1134	WP_121540699.1	amidohydrolase/deacetylase family metallohydrolase	-
CUC47_RS25575	5128110..5129228	+	1119	WP_048234082.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
CUC47_RS25580	5129225..5129965	+	741	WP_048216922.1	KDGP aldolase family protein	-
CUC47_RS25585	5129987..5131900	+	1914	WP_121540700.1	BglG family transcription antiterminator	-
CUC47_RS25590	5131978..5132220	+	243	WP_061548940.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CUC47_RS25595	5132210..5132494	+	285	WP_003839578.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CUC47_RS25600	5132498..5132962	-	465	WP_088902792.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
CUC47_RS25605	5133069..5135207	-	2139	WP_003844922.1	anaerobic ribonucleoside-triphosphate reductase	-
CUC47_RS25610	5135585..5136169	-	585	WP_003839584.1	fructose PTS transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-65)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.68 Da
• Isoelectric Point: 10.0482

>T88317 WP_003839578.1 NZ_CP024679:5132210-5132494 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
REHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T88317 NZ_CP024679:5132210-5132494 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8931.32 Da
• Isoelectric Point: 5.0651

>AT88317 WP_061548940.1 NZ_CP024679:5131978-5132220 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLAGPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT88317 NZ_CP024679:5131978-5132220 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCGGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0D7LMW6