Detailed information of TA system TA088314

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3687941..3688620
• Replicon: chromosome
• Accession: NZ_CP024679
• Organism: Citrobacter freundii strain UMH15

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: CUC47_RS18360
• Protein ID: WP_048217153.1
• Coordinates: 3687941..3688282 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CUC47_RS18365
• Protein ID: WP_048217152.1
• Coordinates: 3688303..3688620 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC47_RS18335	3683094..3684143	+	1050	WP_003838092.1	YncE family protein	-
CUC47_RS18340	3684154..3686151	+	1998	WP_121540465.1	TonB-dependent receptor	-
CUC47_RS18345	3686182..3686688	-	507	WP_003838087.1	G/U mismatch-specific DNA glycosylase	-
CUC47_RS18355	3686992..3687825	-	834	WP_048217154.1	DUF4942 domain-containing protein	-
CUC47_RS18360	3687941..3688282	-	342	WP_048217153.1	TA system toxin CbtA family protein	Toxin
CUC47_RS18365	3688303..3688620	-	318	WP_048217152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CUC47_RS18370	3688639..3688860	-	222	WP_047062281.1	DUF987 domain-containing protein	-
CUC47_RS18375	3688869..3689351	-	483	WP_048217151.1	DNA repair protein RadC	-
CUC47_RS18380	3689360..3689818	-	459	WP_048217150.1	antirestriction protein	-
CUC47_RS18385	3689904..3690140	-	237	WP_048217149.1	DUF905 domain-containing protein	-
CUC47_RS18390	3690218..3690628	-	411	WP_048217148.1	hypothetical protein	-
CUC47_RS18395	3690695..3691132	-	438	WP_048217420.1	hypothetical protein	-
CUC47_RS18400	3691174..3691710	-	537	WP_048217147.1	DUF4339 domain-containing protein	-
CUC47_RS18405	3691736..3692446	-	711	WP_048217146.1	DeoR family transcriptional regulator	-
CUC47_RS18410	3692655..3693479	-	825	WP_048217145.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13010.99 Da
• Isoelectric Point: 9.9961

>T88314 WP_048217153.1 NZ_CP024679:c3688282-3687941 [Citrobacter freundii]
MKPQPATTSRAVKPCLSPVTVWQMLLTRLFEKHYGLTLNDTPFSDETVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQATGLLRQSRNNAVR

Nucleotide

• Length: 342 bp

>T88314 NZ_CP024679:c3688282-3687941 [Citrobacter freundii]
ATGAAACCTCAACCTGCAACAACTTCGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGACAGTCTGGCAAATGCTGCTGAC
CCGATTGTTCGAAAAACACTATGGCCTGACGCTAAACGATACCCCGTTCAGTGATGAAACCGTAATTCAGGAACACATTG
ATGCCGGGATCACGCTGGCCGATGCCGTAAATTTTTTGGTGGAAAAATATGAACTGGTGCGTATCGATCGCAAGGGGTTT
CGCTGGCAGGAACAATCGCCTTATCTTCGGGCTGTAGATATTCTGCGAGCGCGACAGGCAACTGGCTTGTTGCGACAAAG
CCGTAACAATGCAGTACGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11845.30 Da
• Isoelectric Point: 6.6287

>AT88314 WP_048217152.1 NZ_CP024679:c3688620-3688303 [Citrobacter freundii]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASITGKFSDAECRKLDETFPHFISQMESMLTTGEMNPRHAHGVALYH
NGFTCEADTFGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT88314 NZ_CP024679:c3688620-3688303 [Citrobacter freundii]
ATGAGCAACATTACATGGGGCTTGCAGCGGGATACCACGCCGCGTCTTGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGCCATGCCCACGGTGTCGCCCTGTATCAC
AATGGTTTTACCTGCGAAGCCGATACCTTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA