Detailed information of TA system TA088312

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3433871..3434525
• Replicon: chromosome
• Accession: NZ_CP024679
• Organism: Citrobacter freundii strain UMH15

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A0J1NHY7
• Locus tag: CUC47_RS17065
• Protein ID: WP_003026936.1
• Coordinates: 3433871..3434278 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: CUC47_RS17070
• Protein ID: WP_121540428.1
• Coordinates: 3434259..3434525 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC47_RS17045	3429819..3431552	-	1734	WP_121540427.1	single-stranded-DNA-specific exonuclease RecJ	-
CUC47_RS17050	3431558..3432271	-	714	WP_003825520.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
CUC47_RS17055	3432295..3433191	-	897	WP_003026928.1	site-specific tyrosine recombinase XerD	-
CUC47_RS17060	3433305..3433826	+	522	WP_003026933.1	flavodoxin FldB	-
CUC47_RS17065	3433871..3434278	-	408	WP_003026936.1	protein YgfX	Toxin
CUC47_RS17070	3434259..3434525	-	267	WP_121540428.1	FAD assembly factor SdhE	Antitoxin
CUC47_RS17080	3434782..3435762	+	981	WP_003838269.1	tRNA-modifying protein YgfZ	-
CUC47_RS17085	3435856..3436515	-	660	WP_003838267.1	hemolysin III family protein	-
CUC47_RS17090	3436678..3436989	-	312	WP_003026949.1	N(4)-acetylcytidine aminohydrolase	-
CUC47_RS17095	3437042..3437770	+	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
CUC47_RS17100	3437891..3439324	+	1434	WP_048218552.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15953.89 Da
• Isoelectric Point: 11.4054

>T88312 WP_003026936.1 NZ_CP024679:c3434278-3433871 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Nucleotide

• Length: 408 bp

>T88312 NZ_CP024679:c3434278-3433871 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10572.11 Da
• Isoelectric Point: 5.1700

>AT88312 WP_121540428.1 NZ_CP024679:c3434525-3434259 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT88312 NZ_CP024679:c3434525-3434259 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACACCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J1NHY7