Detailed information of TA system TA088303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 588308..588928
• Replicon: chromosome
• Accession: NZ_CP024679
• Organism: Citrobacter freundii strain UMH15

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: CUC47_RS02795
• Protein ID: WP_121539797.1
• Coordinates: 588308..588526 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: R8WZW8
• Locus tag: CUC47_RS02800
• Protein ID: WP_003021733.1
• Coordinates: 588554..588928 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC47_RS02760	583666..584295	+	630	WP_003835929.1	membrane protein	-
CUC47_RS02765	584360..584713	+	354	WP_003831033.1	DUF1428 family protein	-
CUC47_RS02770	584765..586318	-	1554	WP_121539795.1	EAL domain-containing protein	-
CUC47_RS02775	586507..586767	+	261	WP_003021719.1	type B 50S ribosomal protein L31	-
CUC47_RS02780	586769..586909	+	141	WP_005125190.1	type B 50S ribosomal protein L36	-
CUC47_RS02785	586988..587458	-	471	WP_003021724.1	YlaC family protein	-
CUC47_RS02790	587575..588126	-	552	WP_121539796.1	maltose O-acetyltransferase	-
CUC47_RS02795	588308..588526	-	219	WP_121539797.1	hemolysin expression modulator Hha	Toxin
CUC47_RS02800	588554..588928	-	375	WP_003021733.1	Hha toxicity modulator TomB	Antitoxin
CUC47_RS02805	589416..592565	-	3150	WP_003021736.1	multidrug efflux RND transporter permease subunit	-
CUC47_RS02810	592588..593781	-	1194	WP_088902653.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T88303 WP_121539797.1 NZ_CP024679:c588526-588308 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPISVWKFIR

Nucleotide

• Length: 219 bp

>T88303 NZ_CP024679:c588526-588308 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATCCCAATTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14427.23 Da
• Isoelectric Point: 5.5653

>AT88303 WP_003021733.1 NZ_CP024679:c588928-588554 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT88303 NZ_CP024679:c588928-588554 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: R8WZW8