88302

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 338135..338814 Replicon chromosome
Accession NZ_CP024679
Organism Citrobacter freundii strain UMH15

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag CUC47_RS01560 Protein ID WP_003031349.1
Coordinates 338135..338476 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag CUC47_RS01565 Protein ID WP_003031347.1
Coordinates 338497..338814 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC47_RS01535 333879..334610 + 732 WP_044702485.1 winged helix-turn-helix domain-containing protein -
CUC47_RS01540 334585..335058 + 474 WP_003838775.1 hypothetical protein -
CUC47_RS01545 335400..336848 + 1449 WP_003838773.1 EAL domain-containing protein -
CUC47_RS01550 337041..337505 + 465 WP_003843778.1 isoprenylcysteine carboxylmethyltransferase family protein -
CUC47_RS01560 338135..338476 - 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
CUC47_RS01565 338497..338814 - 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CUC47_RS01570 338832..339053 - 222 WP_003031346.1 DUF987 domain-containing protein -
CUC47_RS01575 339062..339538 - 477 WP_003031345.1 RadC family protein -
CUC47_RS01580 339554..340015 - 462 WP_003031344.1 antirestriction protein -
CUC47_RS01585 340029..340220 - 192 Protein_305 DUF905 family protein -
CUC47_RS01590 340724..342721 + 1998 WP_032939120.1 choline BCCT transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 333879..393657 59778


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T88302 WP_003031349.1 NZ_CP024679:c338476-338135 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T88302 NZ_CP024679:c338476-338135 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT88302 WP_003031347.1 NZ_CP024679:c338814-338497 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT88302 NZ_CP024679:c338814-338497 [Citrobacter freundii]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References