Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 5041797..5042313 Replicon chromosome
Accession NZ_CP024677
Organism Citrobacter freundii strain UMH16

Toxin (Protein)


Gene name relE Uniprot ID A0A0J1MTE6
Locus tag CUC48_RS25045 Protein ID WP_016149473.1
Coordinates 5042029..5042313 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0J1MV10
Locus tag CUC48_RS25040 Protein ID WP_003839576.1
Coordinates 5041797..5042039 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC48_RS25020 5036812..5037945 + 1134 WP_016149471.1 amidohydrolase/deacetylase family metallohydrolase -
CUC48_RS25025 5037929..5039047 + 1119 WP_086529650.1 DgaE family pyridoxal phosphate-dependent ammonia lyase -
CUC48_RS25030 5039044..5039784 + 741 WP_003025772.1 KDGP aldolase family protein -
CUC48_RS25035 5039806..5041719 + 1914 WP_086529649.1 BglG family transcription antiterminator -
CUC48_RS25040 5041797..5042039 + 243 WP_003839576.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CUC48_RS25045 5042029..5042313 + 285 WP_016149473.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CUC48_RS25050 5042317..5042781 - 465 WP_032937736.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
CUC48_RS25055 5042888..5045026 - 2139 WP_086529648.1 anaerobic ribonucleoside-triphosphate reductase -
CUC48_RS25060 5045404..5045988 - 585 WP_003839584.1 fructose PTS transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10878.73 Da        Isoelectric Point: 10.0482

>T88301 WP_016149473.1 NZ_CP024677:5042029-5042313 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYLDANKRL

Download         Length: 285 bp

>T88301 NZ_CP024677:5042029-5042313 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.34 Da        Isoelectric Point: 5.0651

>AT88301 WP_003839576.1 NZ_CP024677:5041797-5042039 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Download         Length: 243 bp

>AT88301 NZ_CP024677:5041797-5042039 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MTE6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MV10

References