88300

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4917547..4918100 Replicon chromosome
Accession NZ_CP024677
Organism Citrobacter freundii strain UMH16

Toxin (Protein)


Gene name ccdB Uniprot ID A0A443VE20
Locus tag CUC48_RS24380 Protein ID WP_057108625.1
Coordinates 4917786..4918100 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A443VE25
Locus tag CUC48_RS24375 Protein ID WP_057108464.1
Coordinates 4917547..4917783 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC48_RS24350 4913574..4913924 - 351 WP_009309933.1 IS66 family insertion sequence element accessory protein TnpB -
CUC48_RS24355 4913921..4914325 - 405 WP_009309932.1 IS66 family insertion sequence hypothetical protein -
CUC48_RS24360 4914958..4915176 - 219 Protein_4619 transposase -
CUC48_RS24365 4915109..4915405 - 297 WP_057108462.1 hypothetical protein -
CUC48_RS26155 4916040..4916297 - 258 WP_140415848.1 hypothetical protein -
CUC48_RS24370 4916280..4917224 + 945 WP_057108463.1 DUF1738 domain-containing protein -
CUC48_RS24375 4917547..4917783 + 237 WP_057108464.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CUC48_RS24380 4917786..4918100 + 315 WP_057108625.1 CcdB family protein Toxin
CUC48_RS24385 4918097..4918849 - 753 WP_064388594.1 hypothetical protein -
CUC48_RS24390 4918858..4920594 - 1737 WP_064388593.1 type IV secretory system conjugative DNA transfer family protein -
CUC48_RS24400 4921370..4921675 - 306 WP_072146387.1 dpoa decarboxylase -
CUC48_RS24405 4921724..4922026 - 303 WP_057108600.1 conjugal transfer protein -
CUC48_RS24410 4922075..4922473 - 399 WP_057108599.1 cag pathogenicity island Cag12 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4876822..4937437 60615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(10-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11487.35 Da        Isoelectric Point: 7.9888

>T88300 WP_057108625.1 NZ_CP024677:4917786-4918100 [Citrobacter freundii]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVDRYPAGRRPDRLVPVVRLTDGQEYAVMTHELASVPVRALG
AVFCDASQYRTQVKAAIDFLIDGI

Download         Length: 315 bp

>T88300 NZ_CP024677:4917786-4918100 [Citrobacter freundii]
ATGCAGTTCACCGTATACGGTAATACCGGTAAAAGCGCCGTTTACCCCCTGTTGCTTGATGTCACAAGCGATATCATTGG
ACAACTGAACCGCCGGATCGTGATCCCGTTGTTGCCTGTTGACAGGTATCCGGCAGGCCGCCGCCCGGATCGTCTTGTCC
CCGTGGTCAGACTGACTGACGGCCAGGAATACGCGGTCATGACCCACGAACTGGCGAGTGTCCCTGTCCGGGCGCTGGGG
GCGGTGTTCTGCGATGCGTCGCAGTACCGCACTCAGGTGAAGGCCGCCATCGATTTCCTCATTGACGGCATTTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9012.08 Da        Isoelectric Point: 6.2206

>AT88300 WP_057108464.1 NZ_CP024677:4917547-4917783 [Citrobacter freundii]
MTAKQRNTLSVTMTVERALLVRAREAGINLSATLTAALDAELRHHEAKKWQEENREAIDALNRFHDEYGCFSDEYRTF

Download         Length: 237 bp

>AT88300 NZ_CP024677:4917547-4917783 [Citrobacter freundii]
ATGACCGCGAAACAACGCAACACACTGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAGGCGGG
TATTAACCTGAGTGCGACCCTGACCGCCGCCCTGGATGCGGAGCTTCGTCACCATGAAGCCAAAAAATGGCAGGAAGAGA
ACAGGGAGGCCATCGACGCATTAAACCGTTTCCATGATGAGTATGGCTGTTTCAGTGATGAATACCGGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443VE20

Antitoxin

Source ID Structure
AlphaFold DB A0A443VE25

References