88299

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4877082..4877860 Replicon chromosome
Accession NZ_CP024677
Organism Citrobacter freundii strain UMH16

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A443VCT1
Locus tag CUC48_RS24180 Protein ID WP_057108449.1
Coordinates 4877372..4877860 (+) Length 163 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A1Y0L5F0
Locus tag CUC48_RS24175 Protein ID WP_038917748.1
Coordinates 4877082..4877375 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC48_RS24165 4872785..4874506 + 1722 WP_003844994.1 SulP family inorganic anion transporter -
CUC48_RS24170 4874727..4876388 - 1662 WP_086538682.1 fatty acid--CoA ligase -
CUC48_RS24175 4877082..4877375 + 294 WP_038917748.1 DUF1778 domain-containing protein Antitoxin
CUC48_RS24180 4877372..4877860 + 489 WP_057108449.1 GNAT family N-acetyltransferase Toxin
CUC48_RS24185 4877953..4879056 + 1104 WP_064388374.1 hypothetical protein -
CUC48_RS24190 4879332..4879821 - 490 Protein_4585 molybdopterin-guanine dinucleotide biosynthesis protein MobC -
CUC48_RS24195 4880391..4881800 + 1410 WP_057108451.1 N-6 DNA methylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4876822..4937437 60615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(71-143)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 163 a.a.        Molecular weight: 17572.45 Da        Isoelectric Point: 7.2150

>T88299 WP_057108449.1 NZ_CP024677:4877372-4877860 [Citrobacter freundii]
MISAPEPLRAEHVLSSFCCGVESMDNWLKLRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVAMNSAPGRFRRNMPDPI
PVVMLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SF

Download         Length: 489 bp

>T88299 NZ_CP024677:4877372-4877860 [Citrobacter freundii]
ATGATTTCCGCCCCTGAGCCACTCCGTGCCGAACATGTACTATCTTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTAAAACTACGGGCGATGAAAAATCAGGTCACTGGAGCATCACGAACTTTCGTCAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGTGCTGTAGCAATGAATTCAGCTCCCGGACGTTTTCGCCGAAATATGCCCGATCCTATC
CCGGTCGTGATGTTGGGGCGCTTGGCTGTGGATACGTCATTGCATGGCCAAGGTGTTGGCCGTGCTCTGGTACGTGATGC
TGGGCTAAGAGTGATTCAGGTTGCTGATACTATCGGCATTCGTGGGATGCTGGTTCATGCGCTGTCTGATGAGGCACGGG
AATTTTATCTACGGGTGGGGTTTGAACCTTCGCCGATAGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTTGGG
AGTTTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10872.44 Da        Isoelectric Point: 10.1968

>AT88299 WP_038917748.1 NZ_CP024677:4877082-4877375 [Citrobacter freundii]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT88299 NZ_CP024677:4877082-4877375 [Citrobacter freundii]
ATGCCAGCGACGAACAGCGTTTCTGTTAAACGCGAAACACTGAATTTACGCATCAAACCCGCAGAACGGGATCTGATTGA
TCGTGCAGCTAAGGCCAGAGGAAAAAATCGTACCGATTTTGTCCTTGAAGCTGCTCGCGCTGCCGCGGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGACGCTTATCAGGAATTCCTTGCCCGTTTGGATCAAGCTCCCTCTCCGAAT
GCCGCGCTGCGTAAAACCATGCAAACACCTGCACCGTGGGAACAGAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443VCT1

Antitoxin

Source ID Structure
AlphaFold DB A0A1Y0L5F0

References