Detailed information of TA system TA088295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4148868..4149499
• Replicon: chromosome
• Accession: NZ_CP024677
• Organism: Citrobacter freundii strain UMH16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CUC48_RS20695
• Protein ID: WP_046671263.1
• Coordinates: 4149224..4149499 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0J1MMS0
• Locus tag: CUC48_RS20690
• Protein ID: WP_003837808.1
• Coordinates: 4148868..4149227 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC48_RS20660	4143964..4144629	+	666	WP_003837818.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-
CUC48_RS20665	4144702..4145259	+	558	WP_003837816.1	DcrB family lipoprotein	-
CUC48_RS20670	4145263..4146480	-	1218	WP_016151126.1	MFS transporter	-
CUC48_RS20675	4146614..4147663	+	1050	WP_003023393.1	AI-2E family transporter	-
CUC48_RS20680	4147726..4148304	+	579	WP_071697729.1	4'-phosphopantetheinyl transferase AcpT	-
CUC48_RS20685	4148354..4148755	+	402	WP_003023387.1	nickel-responsive transcriptional regulator NikR	-
CUC48_RS20690	4148868..4149227	-	360	WP_003837808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUC48_RS20695	4149224..4149499	-	276	WP_046671263.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUC48_RS20700	4149577..4150701	-	1125	WP_086538636.1	ABC transporter permease	-
CUC48_RS20705	4150701..4153448	-	2748	WP_049025788.1	ribosome-associated ATPase/putative transporter RbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10238.90 Da
• Isoelectric Point: 10.7844

>T88295 WP_046671263.1 NZ_CP024677:c4149499-4149224 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIRWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T88295 NZ_CP024677:c4149499-4149224 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAGGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13198.98 Da
• Isoelectric Point: 4.5462

>AT88295 WP_003837808.1 NZ_CP024677:c4149227-4148868 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT88295 NZ_CP024677:c4149227-4148868 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A0J1MMS0