Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 3725181..3725860 Replicon chromosome
Accession NZ_CP024677
Organism Citrobacter freundii strain UMH16

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1Z3N0J5
Locus tag CUC48_RS18535 Protein ID WP_045268641.1
Coordinates 3725181..3725522 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A1Z3N0L6
Locus tag CUC48_RS18540 Protein ID WP_045268640.1
Coordinates 3725543..3725860 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC48_RS18515 3721224..3722273 + 1050 WP_003846277.1 YncE family protein -
CUC48_RS18520 3722284..3724281 + 1998 WP_016151004.1 TonB-dependent receptor -
CUC48_RS18525 3724312..3724818 - 507 WP_121581740.1 G/U mismatch-specific DNA glycosylase -
CUC48_RS18535 3725181..3725522 - 342 WP_045268641.1 TA system toxin CbtA family protein Toxin
CUC48_RS18540 3725543..3725860 - 318 WP_045268640.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CUC48_RS18545 3725880..3726101 - 222 WP_007893437.1 DUF987 domain-containing protein -
CUC48_RS18550 3726110..3726586 - 477 WP_121581742.1 RadC family protein -
CUC48_RS18555 3726602..3727060 - 459 WP_045268638.1 antirestriction protein -
CUC48_RS18560 3727162..3727346 - 185 Protein_3538 DUF905 family protein -
CUC48_RS18565 3727735..3729393 + 1659 WP_045268637.1 AAA family ATPase -
CUC48_RS18570 3729386..3730540 + 1155 WP_045268636.1 UvrD-helicase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13045.30 Da        Isoelectric Point: 10.5786

>T88293 WP_045268641.1 NZ_CP024677:c3725522-3725181 [Citrobacter freundii]
MKTLPATTLRVVKPCLSPVAIWKMLLTRLLEQHYGLTLNDTPFSKERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRKNLVR

Download         Length: 342 bp

>T88293 NZ_CP024677:c3725522-3725181 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCTGCGGGTGGTGAAGCCCTGCCTGTCACCCGTGGCTATCTGGAAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTAAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGGTTT
AGCTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTTGGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11976.67 Da        Isoelectric Point: 6.7379

>AT88293 WP_045268640.1 NZ_CP024677:c3725860-3725543 [Citrobacter freundii]
MTHIIWGLQRNITPRLGARLVQEGNRLHYLADRASITGMFSDVECLKLDDTFQHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT88293 NZ_CP024677:c3725860-3725543 [Citrobacter freundii]
ATGACCCACATCATCTGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGACT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGTCGAATGCCTGAAGCTGGATGACACATTCCAGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3N0J5

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3N0L6

References