Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3618606..3619363 Replicon chromosome
Accession NZ_CP024677
Organism Citrobacter freundii strain UMH16

Toxin (Protein)


Gene name tacT Uniprot ID A0A1D8JMF4
Locus tag CUC48_RS18005 Protein ID WP_044159004.1
Coordinates 3618606..3619088 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag CUC48_RS18010 Protein ID WP_007372584.1
Coordinates 3619079..3619363 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC48_RS17985 3613666..3614946 - 1281 WP_176682058.1 type 4b pilus protein PilO2 -
CUC48_RS17990 3614949..3616622 - 1674 WP_013095157.1 PilN family type IVB pilus formation outer membrane protein -
CUC48_RS17995 3616642..3617085 - 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
CUC48_RS18000 3617087..3618373 - 1287 WP_013095156.1 TcpQ domain-containing protein -
CUC48_RS18005 3618606..3619088 - 483 WP_044159004.1 GNAT family N-acetyltransferase Toxin
CUC48_RS18010 3619079..3619363 - 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
CUC48_RS18015 3619519..3620019 - 501 WP_007372583.1 single-stranded DNA-binding protein -
CUC48_RS18020 3620095..3620586 - 492 WP_007372582.1 DUF3577 domain-containing protein -
CUC48_RS18025 3621219..3623231 - 2013 WP_044159002.1 DNA topoisomerase III -
CUC48_RS18030 3623249..3623971 - 723 WP_013095151.1 TIGR03761 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3519137..3631493 112356


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-146)

Antitoxin

Vibrio phage ICP1, Orf50

(1-93)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17693.32 Da        Isoelectric Point: 6.9570

>T88292 WP_044159004.1 NZ_CP024677:c3619088-3618606 [Citrobacter freundii]
MEINVTAPALLTEEHVLHSFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTACVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

>T88292 NZ_CP024677:c3619088-3618606 [Citrobacter freundii]
ATGGAAATAAATGTCACTGCGCCTGCTTTACTGACAGAGGAGCATGTTCTTCATTCGTTCGATTGCGGGAATGCGGTACT
TGATGACTGGCTTCGCCGGCGTGCGTTGAAGAATCAGCATCTTAATGCTTCCCGGACATTCGTCATCTGTCCTGAAGGTA
CTGCGTGTGTAGTGGGTTATTATTCCCTTGCGACGGGCTCAGTTAGTCATGTCGACTTGAGTCGTAGTCTGCGACAGAAT
ATGCCCGATCCCGTACCGGTTGTTTTGCTGGGCCGCCTTGCAGTCGATGTATCCACTCAGGGCAATAACTTCGGGAAATG
GTTGCTGAATGATGCTGTGATGCGCGTATCGAATCTGGCGGATCAGGTCGGCATCAAGGCTATCATGGTGCATGCAATTG
ATGAGCGGGCTAAAGCGTTCTATGAATACTTTGGTTTTGTGCAGTCACCGGTTGCGGCTAACACGCTTTTCTACAAAATA
TAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT88292 WP_007372584.1 NZ_CP024677:c3619363-3619079 [Citrobacter freundii]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp

>AT88292 NZ_CP024677:c3619363-3619079 [Citrobacter freundii]
ATGCAAACTCCAGTCCGTAAATCCGTCCGTGACAAGCAAATCAATATCCGCGCCACGGATGAAGAACGTGCGGTGATTGA
TTACGCTGCGAGTCTGGTTAATAAAAACCGTACTGATTTCATCATTGAAAGGGCTGTGCATGAGGCCCAGAACATCATTC
TGGACCAGCGTGTGTTCGTTCTTGATGATGCCCGTTACCAGGCGTTCATCAGGCAGCTTGAAGCCCCTGTGCAGAATGTT
GAAGGTCGTCAGCGACTGATGGATGTAAAACCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JMF4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References