Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3446084..3446738 Replicon chromosome
Accession NZ_CP024677
Organism Citrobacter freundii strain UMH16

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag CUC48_RS17075 Protein ID WP_003026936.1
Coordinates 3446084..3446491 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag CUC48_RS17080 Protein ID WP_003026938.1
Coordinates 3446472..3446738 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC48_RS17055 3442032..3443765 - 1734 WP_016150943.1 single-stranded-DNA-specific exonuclease RecJ -
CUC48_RS17060 3443771..3444484 - 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CUC48_RS17065 3444508..3445404 - 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
CUC48_RS17070 3445518..3446039 + 522 WP_003026933.1 flavodoxin FldB -
CUC48_RS17075 3446084..3446491 - 408 WP_003026936.1 protein YgfX Toxin
CUC48_RS17080 3446472..3446738 - 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
CUC48_RS17090 3446995..3447975 + 981 WP_086538575.1 tRNA-modifying protein YgfZ -
CUC48_RS17095 3448069..3448728 - 660 WP_003026947.1 hemolysin III family protein -
CUC48_RS17100 3448891..3449202 - 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
CUC48_RS17105 3449255..3449983 + 729 WP_003026952.1 MurR/RpiR family transcriptional regulator -
CUC48_RS17110 3450104..3451537 + 1434 WP_086529872.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T88291 WP_003026936.1 NZ_CP024677:c3446491-3446084 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T88291 NZ_CP024677:c3446491-3446084 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT88291 WP_003026938.1 NZ_CP024677:c3446738-3446472 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88291 NZ_CP024677:c3446738-3446472 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References