Detailed information of TA system TA088282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1828268..1828907
• Replicon: chromosome
• Accession: NZ_CP024677
• Organism: Citrobacter freundii strain UMH16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: CUC48_RS09015
• Protein ID: WP_003020221.1
• Coordinates: 1828731..1828907 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CUC48_RS09010
• Protein ID: WP_123924900.1
• Coordinates: 1828268..1828684 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC48_RS08990	1823314..1824258	-	945	WP_003843727.1	ABC transporter permease	-
CUC48_RS08995	1824259..1825275	-	1017	WP_058842662.1	ABC transporter ATP-binding protein	-
CUC48_RS09000	1825292..1826437	-	1146	WP_061548295.1	ABC transporter substrate-binding protein	-
CUC48_RS09005	1826766..1828175	-	1410	WP_003843728.1	PLP-dependent aminotransferase family protein	-
CUC48_RS09010	1828268..1828684	-	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUC48_RS09015	1828731..1828907	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUC48_RS09020	1829171..1830574	+	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
CUC48_RS09025	1830574..1831584	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
CUC48_RS09030	1831584..1831709	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
CUC48_RS09035	1831899..1832129	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T88282 WP_003020221.1 NZ_CP024677:c1828907-1828731 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T88282 NZ_CP024677:c1828907-1828731 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT88282 WP_123924900.1 NZ_CP024677:c1828684-1828268 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT88282 NZ_CP024677:c1828684-1828268 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCGGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36