Detailed information of TA system TA088279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4596681..4597257
• Replicon: chromosome
• Accession: NZ_CP024676
• Organism: Citrobacter pasteurii strain UMH17

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: CUC49_RS22410
• Protein ID: WP_121585442.1
• Coordinates: 4596681..4596968 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CUC49_RS22415
• Protein ID: WP_121585443.1
• Coordinates: 4596955..4597257 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC49_RS22400	4592745..4595174	-	2430	WP_121585440.1	DEAD/DEAH box helicase family protein	-
CUC49_RS22405	4595270..4596436	-	1167	WP_121585441.1	restriction endonuclease	-
CUC49_RS22410	4596681..4596968	+	288	WP_121585442.1	BrnT family toxin	Toxin
CUC49_RS22415	4596955..4597257	+	303	WP_121585443.1	BrnA antitoxin family protein	Antitoxin
CUC49_RS22420	4597320..4598276	-	957	WP_121585444.1	GTPase	-
CUC49_RS22425	4598289..4598483	-	195	WP_003830038.1	YbdD/YjiX family protein	-
CUC49_RS22430	4598686..4600836	-	2151	WP_121585445.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11187.60 Da
• Isoelectric Point: 7.5234

>T88279 WP_121585442.1 NZ_CP024676:4596681-4596968 [Citrobacter pasteurii]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPQHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVQFESGHEVIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T88279 NZ_CP024676:4596681-4596968 [Citrobacter pasteurii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAAAAAGTAACCTGAGGAAACACGGTGTCCGGTTTGAAGATGCAGT
GCTGGTATTTGACGATCCCCAACATCTGTCGCGGCAGGATCGATATGAGAATGGCGAGTATCGCTGGCAGACCATCGGTC
TTGTTCATGGCATTGTGGTTATTCTGGTTGCCCACAGCGTTCAGTTCGAAAGTGGTCATGAAGTGATTCGCATCATCAGC
GCCAGAAAGGCAGACCGTAAAGAGAGGAGTCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.07 Da
• Isoelectric Point: 9.5646

>AT88279 WP_121585443.1 NZ_CP024676:4596955-4597257 [Citrobacter pasteurii]
MNMVKHKRGSVSTLSPQHEAELKALANKSDDEIDYSDIPATEDEQWSEANRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT88279 NZ_CP024676:4596955-4597257 [Citrobacter pasteurii]
ATGAACATGGTTAAGCATAAACGCGGCAGCGTATCCACGCTGAGTCCTCAGCATGAGGCTGAACTGAAAGCGCTGGCGAA
TAAATCGGATGATGAAATTGATTACAGTGACATTCCTGCGACAGAAGATGAGCAGTGGTCAGAGGCTAATCGCGGTAAGT
TTTTCCGGCCGTTGAAAACGCAGGCATCCGTGCGCATTGATGCTGACGTGATGGAATGGTTGAAACGCCCTGGAAAGGGT
TACCAGACGCGGCTCAACGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG