88260

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4445261..4445877 Replicon chromosome
Accession NZ_CP024675
Organism Citrobacter werkmanii strain UMH18

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CUC50_RS21575 Protein ID WP_085049711.1
Coordinates 4445503..4445877 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A8E9YHE9
Locus tag CUC50_RS21570 Protein ID WP_001523745.1
Coordinates 4445261..4445503 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC50_RS21535 4440671..4441270 + 600 WP_069324890.1 glucose-1-phosphatase -
CUC50_RS21540 4441264..4442136 + 873 WP_085049708.1 virulence factor BrkB family protein -
CUC50_RS21545 4442133..4442570 + 438 WP_003825289.1 D-tyrosyl-tRNA(Tyr) deacylase -
CUC50_RS21550 4442615..4443556 + 942 WP_032940213.1 fatty acid biosynthesis protein FabY -
CUC50_RS21555 4443562..4443849 - 288 WP_121529781.1 type II toxin-antitoxin system RelE/ParE family toxin -
CUC50_RS21560 4443842..4444084 - 243 WP_069324888.1 type II toxin-antitoxin system ParD family antitoxin -
CUC50_RS21565 4444149..4445057 - 909 WP_121529782.1 alpha/beta hydrolase -
CUC50_RS21570 4445261..4445503 + 243 WP_001523745.1 ribbon-helix-helix domain-containing protein Antitoxin
CUC50_RS21575 4445503..4445877 + 375 WP_085049711.1 type II toxin-antitoxin system VapC family toxin Toxin
CUC50_RS21580 4445914..4446843 - 930 WP_121529783.1 formate dehydrogenase accessory protein FdhE -
CUC50_RS21585 4446840..4447475 - 636 WP_038635897.1 formate dehydrogenase cytochrome b556 subunit -
CUC50_RS21590 4447472..4448374 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13697.93 Da        Isoelectric Point: 8.5343

>T88260 WP_085049711.1 NZ_CP024675:4445503-4445877 [Citrobacter werkmanii]
MVKGAALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRIALGAFNIIGVSQDIAERSVNI
RQEYGMKLPDAIILATAQIHHLTLVTRNTKDFASISGVVTPYTL

Download         Length: 375 bp

>T88260 NZ_CP024675:4445503-4445877 [Citrobacter werkmanii]
ATGGTCAAAGGGGCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTGGCGAT
AGAAACATGGCCGCCACAGAACGCAATCAGTCTCATTACATGGATGGAGGTGATGGTAGGCGCAAAAAAATACCATCAGG
AGGCCCGTACTCGCATTGCGCTAGGCGCTTTTAACATTATTGGTGTTTCACAGGATATTGCTGAGCGAAGCGTGAATATC
AGGCAAGAATACGGGATGAAATTGCCGGATGCCATTATCCTGGCTACTGCGCAGATTCATCATCTGACACTGGTTACGCG
TAATACCAAAGATTTTGCAAGCATATCCGGTGTGGTAACGCCGTATACGTTATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.77 Da        Isoelectric Point: 4.4463

>AT88260 WP_001523745.1 NZ_CP024675:4445261-4445503 [Citrobacter werkmanii]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT88260 NZ_CP024675:4445261-4445503 [Citrobacter werkmanii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGACGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAAACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References