Detailed information of TA system TA088259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3955146..3955777
• Replicon: chromosome
• Accession: NZ_CP024675
• Organism: Citrobacter werkmanii strain UMH18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0J1MPQ6
• Locus tag: CUC50_RS19210
• Protein ID: WP_003837806.1
• Coordinates: 3955502..3955777 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CUC50_RS19205
• Protein ID: WP_087856264.1
• Coordinates: 3955146..3955505 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC50_RS19180	3951379..3952428	+	1050	WP_032940622.1	AI-2E family transporter	-
CUC50_RS19185	3952491..3953081	+	591	WP_038635032.1	4'-phosphopantetheinyl transferase AcpT	-
CUC50_RS19190	3953073..3953579	-	507	WP_121529659.1	phenolic acid decarboxylase	-
CUC50_RS19195	3953679..3954539	+	861	WP_038635037.1	LysR family transcriptional regulator	-
CUC50_RS19200	3954631..3955032	+	402	WP_006687486.1	nickel-responsive transcriptional regulator NikR	-
CUC50_RS19205	3955146..3955505	-	360	WP_087856264.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUC50_RS19210	3955502..3955777	-	276	WP_003837806.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUC50_RS19215	3955855..3956979	-	1125	WP_038635040.1	ABC transporter permease	-
CUC50_RS19220	3956979..3959725	-	2747	Protein_3667	ribosome-associated ATPase/putative transporter RbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10210.88 Da
• Isoelectric Point: 10.7228

>T88259 WP_003837806.1 NZ_CP024675:c3955777-3955502 [Citrobacter werkmanii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T88259 NZ_CP024675:c3955777-3955502 [Citrobacter werkmanii]
ATGAAGGAAAAGGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCGCCTACGCCGCAGGG
AATCAAGTGGTCGGAAATCGAGTCACTGATTAAGGCATTAGGTGGCGAAATCAAAGAAGGGCGAGGTTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATCGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13206.02 Da
• Isoelectric Point: 4.7022

>AT88259 WP_087856264.1 NZ_CP024675:c3955505-3955146 [Citrobacter werkmanii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVADSIQGLQKEGEISLREYLDDCSAAGIEPYAHPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT88259 NZ_CP024675:c3955505-3955146 [Citrobacter werkmanii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCTGGGCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTCCGCGGTAAGTTCCTGGGTTTATCAGGCTATTGTGATTTTGTTGCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATTTCCTTACGCGAGTATCTGGATGATTGCTCTGCCGCAGGGATAGAGCCCTATGCTCACCCGGAAAAGCTGAAGACA
TTTACGCTACGCTACCCAGAGTCATTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTGAATACCTG
GATTATCGAAACGCTTAGTAAAAGCCTGAAACAGGCATAG

Structures

Toxin

• AlphaFold DB: A0A0J1MPQ6