Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3360546..3361200 Replicon chromosome
Accession NZ_CP024675
Organism Citrobacter werkmanii strain UMH18

Toxin (Protein)


Gene name cptA Uniprot ID A0A2Z3X2B8
Locus tag CUC50_RS16175 Protein ID WP_038633481.1
Coordinates 3360546..3360953 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID R8WMJ6
Locus tag CUC50_RS16180 Protein ID WP_016154349.1
Coordinates 3360934..3361200 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC50_RS16155 3356438..3358171 - 1734 WP_069324822.1 single-stranded-DNA-specific exonuclease RecJ -
CUC50_RS16160 3358177..3358890 - 714 WP_061382414.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CUC50_RS16165 3358914..3359810 - 897 WP_038633475.1 site-specific tyrosine recombinase XerD -
CUC50_RS16170 3359924..3360445 + 522 WP_038633478.1 flavodoxin FldB -
CUC50_RS16175 3360546..3360953 - 408 WP_038633481.1 protein YgfX Toxin
CUC50_RS16180 3360934..3361200 - 267 WP_016154349.1 FAD assembly factor SdhE Antitoxin
CUC50_RS16190 3361457..3362437 + 981 WP_069324821.1 tRNA-modifying protein YgfZ -
CUC50_RS16195 3362529..3363188 - 660 WP_121529522.1 hemolysin III family protein -
CUC50_RS16200 3363352..3363663 - 312 WP_085048763.1 N(4)-acetylcytidine aminohydrolase -
CUC50_RS16205 3363716..3364444 + 729 WP_085048762.1 MurR/RpiR family transcriptional regulator -
CUC50_RS16210 3364565..3365993 + 1429 Protein_3096 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15944.81 Da        Isoelectric Point: 11.2845

>T88258 WP_038633481.1 NZ_CP024675:c3360953-3360546 [Citrobacter werkmanii]
VVQWQSDLRVSWRAQWLSLLVHGLVAVFILLMPWPLSYTPLWMVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIVSAPWMVKTGMMLRLRSDTGKRQHLWLAADSMDEAEWRDLRRLMLQQAKQG

Download         Length: 408 bp

>T88258 NZ_CP024675:c3360953-3360546 [Citrobacter werkmanii]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAGTGGCTTTCGTTGCTCGTTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCTTTGAGCTACACCCCATTATGGATGGTTCTGCTCTCTCTGGTGGTGTTCGACAGCG
TACGTAGCCAACGTAGAATTAACGCCTGTCAGGGCGAGATTAGGCTATTGATGGACGGCCGTCTGCGCTGGCAAGGGCAG
GAGTGGACTATCGTTAGCGCGCCCTGGATGGTCAAAACAGGCATGATGCTGCGTTTGCGTTCAGATACCGGGAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGAGGCTGAGTGGCGAGATTTACGGCGGTTAATGCTGCAACAGGCGAAGC
AGGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10557.10 Da        Isoelectric Point: 5.6375

>AT88258 WP_016154349.1 NZ_CP024675:c3361200-3360934 [Citrobacter werkmanii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88258 NZ_CP024675:c3361200-3360934 [Citrobacter werkmanii]
ATGGATATTAACAATAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCTATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGACGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTCAGTTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3X2B8

Antitoxin

Source ID Structure
AlphaFold DB R8WMJ6

References