Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 542686..543306 Replicon chromosome
Accession NZ_CP024675
Organism Citrobacter werkmanii strain UMH18

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CUC50_RS02515 Protein ID WP_002892050.1
Coordinates 542686..542904 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag CUC50_RS02520 Protein ID WP_003021733.1
Coordinates 542932..543306 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC50_RS02480 538045..538674 + 630 WP_087857057.1 hypothetical protein -
CUC50_RS02485 538739..539092 + 354 WP_042310059.1 DUF1428 family protein -
CUC50_RS02490 539144..540697 - 1554 WP_085047627.1 EAL domain-containing protein -
CUC50_RS02495 540886..541146 + 261 WP_038638331.1 type B 50S ribosomal protein L31 -
CUC50_RS02500 541148..541288 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
CUC50_RS02505 541362..541832 - 471 WP_069323967.1 YlaC family protein -
CUC50_RS02510 541949..542500 - 552 WP_121528689.1 maltose O-acetyltransferase -
CUC50_RS02515 542686..542904 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CUC50_RS02520 542932..543306 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
CUC50_RS02525 543794..546943 - 3150 WP_038638345.1 multidrug efflux RND transporter permease subunit -
CUC50_RS02530 546966..548159 - 1194 WP_038638348.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T88248 WP_002892050.1 NZ_CP024675:c542904-542686 [Citrobacter werkmanii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T88248 NZ_CP024675:c542904-542686 [Citrobacter werkmanii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGCTGCCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCGGATCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAGATTCCAACTTCCGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT88248 WP_003021733.1 NZ_CP024675:c543306-542932 [Citrobacter werkmanii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT88248 NZ_CP024675:c543306-542932 [Citrobacter werkmanii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAACGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References