Detailed information of TA system TA088247

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4902100..4902779
• Replicon: chromosome
• Accession: NZ_CP024673
• Organism: Citrobacter freundii strain UMH19

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: CUC51_RS24425
• Protein ID: WP_098992213.1
• Coordinates: 4902438..4902779 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CUC51_RS24420
• Protein ID: WP_121567137.1
• Coordinates: 4902100..4902417 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC51_RS24375	4897243..4898067	+	825	WP_121567129.1	DUF945 domain-containing protein	-
CUC51_RS24380	4898275..4898985	+	711	WP_121567130.1	DeoR family transcriptional regulator	-
CUC51_RS24385	4899011..4899547	+	537	WP_121567131.1	DUF4339 domain-containing protein	-
CUC51_RS24390	4899589..4900026	+	438	WP_023301392.1	hypothetical protein	-
CUC51_RS24395	4900093..4900503	+	411	WP_121567132.1	hypothetical protein	-
CUC51_RS24400	4900580..4900816	+	237	WP_121567133.1	DUF905 domain-containing protein	-
CUC51_RS24405	4900902..4901360	+	459	WP_121567134.1	antirestriction protein	-
CUC51_RS24410	4901369..4901851	+	483	WP_121567135.1	DNA repair protein RadC	-
CUC51_RS24415	4901860..4902081	+	222	WP_121567136.1	DUF987 domain-containing protein	-
CUC51_RS24420	4902100..4902417	+	318	WP_121567137.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CUC51_RS24425	4902438..4902779	+	342	WP_098992213.1	TA system toxin CbtA family protein	Toxin
CUC51_RS24430	4903212..4903538	-	327	WP_121567138.1	hypothetical protein	-
CUC51_RS24435	4903866..4904738	-	873	WP_081368679.1	HNH endonuclease	-
CUC51_RS24440	4904879..4905514	+	636	WP_003837147.1	HNH endonuclease	-
CUC51_RS24445	4905566..4905928	-	363	WP_071696540.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4871582..4903538	31956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12879.91 Da
• Isoelectric Point: 8.9786

>T88247 WP_098992213.1 NZ_CP024673:4902438-4902779 [Citrobacter freundii]
MKPQPATTSRAVKPCLSPVAVWQMLLTRLLEQHYGLKMNDTPFSDETVIKEHIDAGITLADAVNFLVDKYELVRIDRRGF
SWQEQSPYLRAVDILRARQATGLLRRSLNDAVL

Nucleotide

• Length: 342 bp

>T88247 NZ_CP024673:4902438-4902779 [Citrobacter freundii]
ATGAAACCTCAACCTGCAACAACTTCGCGGGCGGTAAAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGCCTGAAAATGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATTG
ATGCCGGTATCACGCTGGCTGATGCCGTTAACTTTCTGGTGGATAAGTACGAGCTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCACGACAGGCAACTGGCTTGTTACGACGAAG
TCTTAATGATGCTGTATTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11841.49 Da
• Isoelectric Point: 6.7382

>AT88247 WP_121567137.1 NZ_CP024673:4902100-4902417 [Citrobacter freundii]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRVSFTGTFSEAECLKLDVAFPHFISQMESMLTTGELNPRHAHCVTLYH
NSFICEADTLGSCGYVHIAIYPAQR

Nucleotide

• Length: 318 bp

>AT88247 NZ_CP024673:4902100-4902417 [Citrobacter freundii]
ATGAGCAACATCACATGGGGCCTACAGCGAGATATCACACCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGTCAGTTTCACCGGTACGTTCAGCGAAGCTGAATGTCTTAAGCTGGATGTGGCATTTCCGC
ATTTTATCAGTCAGATGGAATCAATGCTGACCACTGGTGAACTGAATCCCCGTCATGCCCACTGCGTCACCCTGTACCAC
AACAGTTTCATCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTACACATCGCCATTTACCCCGCTCAGCGTTAA