Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4391774..4392390 Replicon chromosome
Accession NZ_CP024673
Organism Citrobacter freundii strain UMH19

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag CUC51_RS21850 Protein ID WP_003028682.1
Coordinates 4392016..4392390 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag CUC51_RS21845 Protein ID WP_043018956.1
Coordinates 4391774..4392016 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC51_RS21815 4387624..4388223 + 600 WP_003028663.1 glucose-1-phosphatase -
CUC51_RS21820 4388217..4389089 + 873 WP_003028669.1 virulence factor BrkB family protein -
CUC51_RS21825 4389086..4389523 + 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
CUC51_RS21830 4389568..4390509 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
CUC51_RS21840 4390660..4391568 - 909 WP_071696216.1 alpha/beta hydrolase -
CUC51_RS21845 4391774..4392016 + 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
CUC51_RS21850 4392016..4392390 + 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
CUC51_RS21855 4392427..4393356 - 930 WP_071696217.1 formate dehydrogenase accessory protein FdhE -
CUC51_RS21860 4393353..4393988 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
CUC51_RS21865 4393985..4394887 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T88244 WP_003028682.1 NZ_CP024673:4392016-4392390 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T88244 NZ_CP024673:4392016-4392390 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT88244 WP_043018956.1 NZ_CP024673:4391774-4392016 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT88244 NZ_CP024673:4391774-4392016 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References