Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 3473784..3474463 Replicon chromosome
Accession NZ_CP024673
Organism Citrobacter freundii strain UMH19

Toxin (Protein)


Gene name ykfI Uniprot ID A0A7D6Z078
Locus tag CUC51_RS17225 Protein ID WP_048215514.1
Coordinates 3473784..3474125 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0F6RHY7
Locus tag CUC51_RS17230 Protein ID WP_046495841.1
Coordinates 3474146..3474463 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC51_RS17200 3468937..3469986 + 1050 WP_071696308.1 YncE family protein -
CUC51_RS17205 3469997..3471994 + 1998 WP_071696309.1 TonB-dependent receptor -
CUC51_RS17210 3472025..3472531 - 507 WP_071696310.1 G/U mismatch-specific DNA glycosylase -
CUC51_RS17220 3472835..3473668 - 834 WP_048215515.1 DUF4942 domain-containing protein -
CUC51_RS17225 3473784..3474125 - 342 WP_048215514.1 TA system toxin CbtA family protein Toxin
CUC51_RS17230 3474146..3474463 - 318 WP_046495841.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CUC51_RS17235 3474482..3474703 - 222 WP_000691995.1 DUF987 domain-containing protein -
CUC51_RS17240 3474712..3475188 - 477 WP_046495838.1 RadC family protein -
CUC51_RS17245 3475204..3475662 - 459 WP_046495834.1 antirestriction protein -
CUC51_RS17250 3475741..3475977 - 237 WP_046495830.1 DUF905 domain-containing protein -
CUC51_RS17255 3476055..3476465 - 411 WP_046495825.1 hypothetical protein -
CUC51_RS17260 3476554..3477168 - 615 WP_046495819.1 hypothetical protein -
CUC51_RS17265 3477165..3477869 - 705 WP_046495813.1 WYL domain-containing protein -
CUC51_RS17270 3478071..3478904 - 834 WP_103856759.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3472835..3506729 33894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(2-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13014.97 Da        Isoelectric Point: 10.4182

>T88242 WP_048215514.1 NZ_CP024673:c3474125-3473784 [Citrobacter freundii]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T88242 NZ_CP024673:c3474125-3473784 [Citrobacter freundii]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11869.49 Da        Isoelectric Point: 6.6245

>AT88242 WP_046495841.1 NZ_CP024673:c3474463-3474146 [Citrobacter freundii]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Download         Length: 318 bp

>AT88242 NZ_CP024673:c3474463-3474146 [Citrobacter freundii]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6Z078

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RHY7

References