Detailed information of TA system TA088232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1770818..1771457
• Replicon: chromosome
• Accession: NZ_CP024673
• Organism: Citrobacter freundii strain UMH19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: CUC51_RS08805
• Protein ID: WP_003020221.1
• Coordinates: 1771281..1771457 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CUC51_RS08800
• Protein ID: WP_128298791.1
• Coordinates: 1770818..1771234 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC51_RS08780	1765865..1766809	-	945	WP_003843727.1	ABC transporter permease	-
CUC51_RS08785	1766810..1767826	-	1017	WP_071696128.1	ABC transporter ATP-binding protein	-
CUC51_RS08790	1767843..1768988	-	1146	WP_061548295.1	ABC transporter substrate-binding protein	-
CUC51_RS08795	1769316..1770725	-	1410	WP_071696130.1	PLP-dependent aminotransferase family protein	-
CUC51_RS08800	1770818..1771234	-	417	WP_128298791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUC51_RS08805	1771281..1771457	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUC51_RS08810	1771721..1773124	+	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
CUC51_RS08815	1773124..1774134	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
CUC51_RS08820	1774134..1774259	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
CUC51_RS08825	1774449..1774679	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T88232 WP_003020221.1 NZ_CP024673:c1771457-1771281 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T88232 NZ_CP024673:c1771457-1771281 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15154.42 Da
• Isoelectric Point: 4.7238

>AT88232 WP_128298791.1 NZ_CP024673:c1771234-1770818 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAARAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT88232 NZ_CP024673:c1771234-1770818 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACTCCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCGGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCGGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36