88230

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 330523..331202 Replicon chromosome
Accession NZ_CP024673
Organism Citrobacter freundii strain UMH19

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag CUC51_RS01530 Protein ID WP_003031349.1
Coordinates 330523..330864 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag CUC51_RS01535 Protein ID WP_003031347.1
Coordinates 330885..331202 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC51_RS01505 325772..326173 + 402 WP_071696410.1 sigma factor-binding protein Crl -
CUC51_RS01510 326229..327284 - 1056 WP_003031375.1 phosphoporin PhoE -
CUC51_RS01515 327575..328678 + 1104 WP_003031373.1 glutamate 5-kinase -
CUC51_RS01520 328690..329943 + 1254 WP_071696411.1 glutamate-5-semialdehyde dehydrogenase -
CUC51_RS01530 330523..330864 - 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
CUC51_RS01535 330885..331202 - 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CUC51_RS01540 331220..331441 - 222 WP_003031346.1 DUF987 domain-containing protein -
CUC51_RS01545 331450..331926 - 477 WP_121566968.1 RadC family protein -
CUC51_RS01550 331942..332403 - 462 WP_003031344.1 antirestriction protein -
CUC51_RS01555 332417..332608 - 192 Protein_301 DUF905 family protein -
CUC51_RS01560 332966..333946 + 981 WP_121566969.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 332966..333946 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T88230 WP_003031349.1 NZ_CP024673:c330864-330523 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T88230 NZ_CP024673:c330864-330523 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT88230 WP_003031347.1 NZ_CP024673:c331202-330885 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT88230 NZ_CP024673:c331202-330885 [Citrobacter freundii]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References