Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4748442..4748958 Replicon chromosome
Accession NZ_CP024672
Organism Citrobacter freundii strain HM38

Toxin (Protein)


Gene name relE Uniprot ID A0A133L8K5
Locus tag CUC52_RS23380 Protein ID WP_003844927.1
Coordinates 4748674..4748958 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0J1MV10
Locus tag CUC52_RS23375 Protein ID WP_003839576.1
Coordinates 4748442..4748684 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC52_RS23355 4743457..4744590 + 1134 WP_060855415.1 amidohydrolase/deacetylase family metallohydrolase -
CUC52_RS23360 4744574..4745692 + 1119 WP_048216923.1 DgaE family pyridoxal phosphate-dependent ammonia lyase -
CUC52_RS23365 4745689..4746429 + 741 WP_048216922.1 KDGP aldolase family protein -
CUC52_RS23370 4746451..4748364 + 1914 WP_003844930.1 BglG family transcription antiterminator -
CUC52_RS23375 4748442..4748684 + 243 WP_003839576.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CUC52_RS23380 4748674..4748958 + 285 WP_003844927.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CUC52_RS23385 4748962..4749426 - 465 WP_032937736.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
CUC52_RS23390 4749533..4751671 - 2139 WP_003844922.1 anaerobic ribonucleoside-triphosphate reductase -
CUC52_RS23395 4752080..4753732 - 1653 WP_016149475.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10892.76 Da        Isoelectric Point: 10.0482

>T88229 WP_003844927.1 NZ_CP024672:4748674-4748958 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLLYQVRDEVVIVFVVAVGK
REHSAVYLDANKRL

Download         Length: 285 bp

>T88229 NZ_CP024672:4748674-4748958 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGCTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.34 Da        Isoelectric Point: 5.0651

>AT88229 WP_003839576.1 NZ_CP024672:4748442-4748684 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Download         Length: 243 bp

>AT88229 NZ_CP024672:4748442-4748684 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTTGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133L8K5

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MV10

References