Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3929576..3930207 Replicon chromosome
Accession NZ_CP024672
Organism Citrobacter freundii strain HM38

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag CUC52_RS19435 Protein ID WP_003837806.1
Coordinates 3929932..3930207 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag CUC52_RS19430 Protein ID WP_003837808.1
Coordinates 3929576..3929935 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC52_RS19400 3924672..3925337 + 666 WP_003837818.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -
CUC52_RS19405 3925410..3925967 + 558 WP_003837816.1 DcrB family lipoprotein -
CUC52_RS19410 3925971..3927188 - 1218 WP_003846164.1 MFS transporter -
CUC52_RS19415 3927322..3928371 + 1050 WP_003023393.1 AI-2E family transporter -
CUC52_RS19420 3928434..3929012 + 579 WP_003023390.1 4'-phosphopantetheinyl transferase AcpT -
CUC52_RS19425 3929062..3929463 + 402 WP_003023387.1 nickel-responsive transcriptional regulator NikR -
CUC52_RS19430 3929576..3929935 - 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CUC52_RS19435 3929932..3930207 - 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
CUC52_RS19440 3930285..3931409 - 1125 WP_032950438.1 ABC transporter permease -
CUC52_RS19445 3931409..3934156 - 2748 WP_032937231.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T88226 WP_003837806.1 NZ_CP024672:c3930207-3929932 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T88226 NZ_CP024672:c3930207-3929932 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT88226 WP_003837808.1 NZ_CP024672:c3929935-3929576 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT88226 NZ_CP024672:c3929935-3929576 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References