Detailed information of TA system TA088225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3904763..3905349
• Replicon: chromosome
• Accession: NZ_CP024672
• Organism: Citrobacter freundii strain HM38

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0J1QS94
• Locus tag: CUC52_RS19280
• Protein ID: WP_032937237.1
• Coordinates: 3904763..3905131 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0J1N092
• Locus tag: CUC52_RS19285
• Protein ID: WP_032937236.1
• Coordinates: 3905128..3905349 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC52_RS19260	3900301..3901371	-	1071	WP_003837845.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
CUC52_RS19265	3901374..3902219	-	846	WP_003023450.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
CUC52_RS19270	3902216..3903103	-	888	WP_032937238.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
CUC52_RS19275	3903204..3904520	-	1317	WP_003023445.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
CUC52_RS19280	3904763..3905131	-	369	WP_032937237.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CUC52_RS19285	3905128..3905349	-	222	WP_032937236.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CUC52_RS19290	3905471..3905752	-	282	WP_003023442.1	hypothetical protein	-
CUC52_RS19295	3905855..3906568	-	714	WP_003827581.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
CUC52_RS19300	3906586..3907353	-	768	WP_003023438.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
CUC52_RS19305	3907350..3908627	-	1278	WP_032950432.1	branched chain amino acid ABC transporter permease LivM	-
CUC52_RS19310	3908624..3909550	-	927	WP_003023435.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3899561..3908627	9066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.91 Da
• Isoelectric Point: 6.9891

>T88225 WP_032937237.1 NZ_CP024672:c3905131-3904763 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIVLSANPDFVAMTVEAAAGQLTLEQIAHRLRH

Nucleotide

• Length: 369 bp

>T88225 NZ_CP024672:c3905131-3904763 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8306.41 Da
• Isoelectric Point: 4.7906

>AT88225 WP_032937236.1 NZ_CP024672:c3905349-3905128 [Citrobacter freundii]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT88225 NZ_CP024672:c3905349-3905128 [Citrobacter freundii]
ATGCGCACTATGAACTATAGTGAAGCTCGACAAAACCTGGCTGAAGTGCTGGAGAGCGCTGTCACTGGTGTGCCTGTTAC
CATTACGCGCCGTGGTCATAAACCTGCGGTAATCATCAGCGCGGAAGAGTTTGAACGCTATCAGGCCGCCAGACTTGATG
ATGAATTTGCCGACATAATGATGATTCATGGTAATGAAATCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0J1QS94

Antitoxin

• AlphaFold DB: A0A0J1N092