Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3356168..3356822 Replicon chromosome
Accession NZ_CP024672
Organism Citrobacter freundii strain HM38

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag CUC52_RS16485 Protein ID WP_003026936.1
Coordinates 3356168..3356575 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag CUC52_RS16490 Protein ID WP_003026938.1
Coordinates 3356556..3356822 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC52_RS16465 3352116..3353849 - 1734 WP_060854985.1 single-stranded-DNA-specific exonuclease RecJ -
CUC52_RS16470 3353855..3354568 - 714 WP_003026923.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CUC52_RS16475 3354592..3355488 - 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
CUC52_RS16480 3355602..3356123 + 522 WP_003026933.1 flavodoxin FldB -
CUC52_RS16485 3356168..3356575 - 408 WP_003026936.1 protein YgfX Toxin
CUC52_RS16490 3356556..3356822 - 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
CUC52_RS16500 3357079..3358059 + 981 WP_003026944.1 tRNA-modifying protein YgfZ -
CUC52_RS16505 3358153..3358812 - 660 WP_003838267.1 hemolysin III family protein -
CUC52_RS16510 3358975..3359286 - 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
CUC52_RS16515 3359339..3360067 + 729 WP_060855085.1 MurR/RpiR family transcriptional regulator -
CUC52_RS16520 3360188..3361621 + 1434 WP_048218552.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T88224 WP_003026936.1 NZ_CP024672:c3356575-3356168 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T88224 NZ_CP024672:c3356575-3356168 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCTTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT88224 WP_003026938.1 NZ_CP024672:c3356822-3356556 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88224 NZ_CP024672:c3356822-3356556 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References