Detailed information of TA system TA088215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1776801..1777440
• Replicon: chromosome
• Accession: NZ_CP024672
• Organism: Citrobacter freundii strain HM38

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: CUC52_RS08625
• Protein ID: WP_003020221.1
• Coordinates: 1777264..1777440 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CUC52_RS08620
• Protein ID: WP_123924900.1
• Coordinates: 1776801..1777217 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC52_RS08600	1771847..1772791	-	945	WP_003843727.1	ABC transporter permease	-
CUC52_RS08605	1772792..1773808	-	1017	WP_058842662.1	ABC transporter ATP-binding protein	-
CUC52_RS08610	1773825..1774970	-	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
CUC52_RS08615	1775299..1776708	-	1410	WP_003843728.1	PLP-dependent aminotransferase family protein	-
CUC52_RS08620	1776801..1777217	-	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CUC52_RS08625	1777264..1777440	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
CUC52_RS08630	1777704..1779107	+	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
CUC52_RS08635	1779107..1780117	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
CUC52_RS08640	1780117..1780242	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
CUC52_RS08645	1780432..1780662	+	231	WP_048217543.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T88215 WP_003020221.1 NZ_CP024672:c1777440-1777264 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T88215 NZ_CP024672:c1777440-1777264 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT88215 WP_123924900.1 NZ_CP024672:c1777217-1776801 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT88215 NZ_CP024672:c1777217-1776801 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCAGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36