Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1241821..1242411 Replicon chromosome
Accession NZ_CP024672
Organism Citrobacter freundii strain HM38

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag CUC52_RS05880 Protein ID WP_003836692.1
Coordinates 1242079..1242411 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag CUC52_RS05875 Protein ID WP_003836694.1
Coordinates 1241821..1242078 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC52_RS05865 1237308..1241207 - 3900 WP_060855256.1 hypothetical protein -
CUC52_RS05875 1241821..1242078 + 258 WP_003836694.1 hypothetical protein Antitoxin
CUC52_RS05880 1242079..1242411 + 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CUC52_RS05890 1242886..1243803 + 918 WP_054528287.1 nitrogen assimilation transcriptional regulator NAC -
CUC52_RS05895 1243905..1244855 + 951 WP_016149950.1 HTH-type transcriptional regulator Cbl -
CUC52_RS05905 1245171..1246658 - 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1231787..1253844 22057


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T88214 WP_003836692.1 NZ_CP024672:1242079-1242411 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T88214 NZ_CP024672:1242079-1242411 [Citrobacter freundii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTTTCTCCAGCATCATTTAATAAGTTCACCCGGCTACCTGTTGTCGTTCCCGTCACTAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGTGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCTCGGAACGGTAAGTGTCTGGAACGTATACCCGAAGCTGTTGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT88214 WP_003836694.1 NZ_CP024672:1241821-1242078 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT88214 NZ_CP024672:1241821-1242078 [Citrobacter freundii]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCTGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References