Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 538033..538653 Replicon chromosome
Accession NZ_CP024672
Organism Citrobacter freundii strain HM38

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CUC52_RS02475 Protein ID WP_002892050.1
Coordinates 538033..538251 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag CUC52_RS02480 Protein ID WP_003021733.1
Coordinates 538279..538653 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC52_RS02440 533266..533895 + 630 WP_003021707.1 membrane protein -
CUC52_RS02445 533960..534313 + 354 WP_003831033.1 DUF1428 family protein -
CUC52_RS02450 534365..535918 - 1554 WP_003835927.1 EAL domain-containing protein -
CUC52_RS02455 536107..536367 + 261 WP_003021719.1 type B 50S ribosomal protein L31 -
CUC52_RS02460 536369..536509 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
CUC52_RS02465 536588..537058 - 471 WP_003021724.1 YlaC family protein -
CUC52_RS02470 537175..537726 - 552 WP_032936967.1 maltose O-acetyltransferase -
CUC52_RS02475 538033..538251 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CUC52_RS02480 538279..538653 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
CUC52_RS02485 539141..542290 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
CUC52_RS02490 542313..543506 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T88213 WP_002892050.1 NZ_CP024672:c538251-538033 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T88213 NZ_CP024672:c538251-538033 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT88213 WP_003021733.1 NZ_CP024672:c538653-538279 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT88213 NZ_CP024672:c538653-538279 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References