Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 78609..79252 Replicon plasmid pRM9872-C1
Accession NZ_CP024660
Organism Escherichia coli strain RM9872-C1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag CUB84_RS26670 Protein ID WP_001044768.1
Coordinates 78609..79025 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag CUB84_RS26675 Protein ID WP_001261287.1
Coordinates 79022..79252 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB84_RS26640 73845..74192 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
CUB84_RS26645 74189..74863 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
CUB84_RS26650 74917..75303 - 387 WP_001066949.1 tyrosine-type recombinase/integrase -
CUB84_RS26655 75431..76291 + 861 WP_000704534.1 alpha/beta hydrolase -
CUB84_RS26665 77902..78447 + 546 WP_001165114.1 hypothetical protein -
CUB84_RS26670 78609..79025 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
CUB84_RS26675 79022..79252 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CUB84_RS26680 79812..80225 + 414 WP_000465041.1 hypothetical protein -
CUB84_RS26685 80227..81009 + 783 WP_001164205.1 site-specific integrase -
CUB84_RS26690 81182..81535 + 354 WP_000864810.1 colicin M immunity protein -
CUB84_RS26695 81543..81734 - 192 Protein_82 lipid II-degrading bacteriocin -
CUB84_RS26700 81738..81881 - 144 Protein_83 transcriptional regulator -
CUB84_RS26705 81948..83387 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - espP / toxB / toxB / hlyD / hlyB / hlyA / hlyC 1..89518 89518


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T88212 WP_001044768.1 NZ_CP024660:c79025-78609 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T88212 NZ_CP024660:c79025-78609 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT88212 WP_001261287.1 NZ_CP024660:c79252-79022 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT88212 NZ_CP024660:c79252-79022 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References