Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1759736..1760374 Replicon chromosome
Accession NZ_CP024659
Organism Escherichia coli strain RM9872-C1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CUB84_RS08685 Protein ID WP_000813794.1
Coordinates 1759736..1759912 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CUB84_RS08690 Protein ID WP_001270286.1
Coordinates 1759958..1760374 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB84_RS08665 1755358..1756569 - 1212 WP_071792349.1 BenE family transporter YdcO -
CUB84_RS08670 1756622..1757158 + 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
CUB84_RS08675 1757231..1759192 + 1962 WP_000218823.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CUB84_RS08680 1759284..1759514 - 231 WP_000494244.1 YncJ family protein -
CUB84_RS08685 1759736..1759912 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CUB84_RS08690 1759958..1760374 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CUB84_RS08695 1760453..1761859 + 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
CUB84_RS08700 1762105..1763250 + 1146 WP_000335342.1 ABC transporter substrate-binding protein -
CUB84_RS08705 1763268..1764281 + 1014 WP_000220414.1 ABC transporter ATP-binding protein -
CUB84_RS08710 1764282..1765223 + 942 WP_001251328.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T88198 WP_000813794.1 NZ_CP024659:1759736-1759912 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T88198 NZ_CP024659:1759736-1759912 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT88198 WP_001270286.1 NZ_CP024659:1759958-1760374 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT88198 NZ_CP024659:1759958-1760374 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References