Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5483210..5483852 Replicon chromosome
Accession NZ_CP024655
Organism Bacillus cereus strain MLY1

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag CSW12_RS28705 Protein ID WP_000635965.1
Coordinates 5483502..5483852 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag CSW12_RS28700 Protein ID WP_000004570.1
Coordinates 5483210..5483497 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSW12_RS28675 5478537..5479489 + 953 Protein_5399 UV DNA damage repair endonuclease UvsE -
CSW12_RS28680 5479482..5480054 - 573 WP_000908526.1 rhomboid family intramembrane serine protease -
CSW12_RS28685 5480148..5480507 + 360 WP_000583418.1 holo-ACP synthase -
CSW12_RS28690 5480664..5481614 + 951 WP_139213356.1 outer membrane lipoprotein carrier protein LolA -
CSW12_RS28695 5481732..5482901 + 1170 WP_000390593.1 alanine racemase -
CSW12_RS28700 5483210..5483497 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
CSW12_RS28705 5483502..5483852 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CSW12_RS28710 5483920..5486088 + 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
CSW12_RS28715 5486146..5486262 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
CSW12_RS28720 5486458..5486916 + 459 WP_000503730.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T88187 WP_000635965.1 NZ_CP024655:5483502-5483852 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T88187 NZ_CP024655:5483502-5483852 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CAAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT88187 WP_000004570.1 NZ_CP024655:5483210-5483497 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT88187 NZ_CP024655:5483210-5483497 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References