Detailed information of TA system TA088186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3049387..3050365
• Replicon: chromosome
• Accession: NZ_CP024655
• Organism: Bacillus cereus strain MLY1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CSW12_RS15650
• Protein ID: WP_053564491.1
• Coordinates: 3049628..3050365 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: CSW12_RS15645
• Protein ID: WP_000237818.1
• Coordinates: 3049387..3049515 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSW12_RS15610	3046044..3046433	+	390	WP_100910266.1	YxeA family protein	-
CSW12_RS15615	3046453..3046629	-	177	WP_100910267.1	stage II sporulation protein SB	-
CSW12_RS15625	3046957..3047937	+	981	WP_090976589.1	WXG100 family type VII secretion target	-
CSW12_RS31635	3047998..3048792	+	795	WP_176518896.1	hypothetical protein	-
CSW12_RS15635	3048819..3049094	+	276	WP_063547286.1	hypothetical protein	-
CSW12_RS31640	3049138..3049314	-	177	WP_090976585.1	hypothetical protein	-
CSW12_RS15645	3049387..3049515	-	129	WP_000237818.1	hypothetical protein	Antitoxin
CSW12_RS15650	3049628..3050365	-	738	WP_053564491.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CSW12_RS15660	3050604..3051341	-	738	WP_053564492.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CSW12_RS15665	3051508..3051990	-	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
CSW12_RS15670	3052098..3053783	+	1686	WP_098289802.1	alpha-keto acid decarboxylase family protein	-
CSW12_RS15675	3053941..3054723	-	783	WP_098836102.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.3184

>T88186 WP_053564491.1 NZ_CP024655:c3050365-3049628 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFFWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T88186 NZ_CP024655:c3050365-3049628 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTTTTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACTGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTGATGTTTACTGCGATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT88186 WP_000237818.1 NZ_CP024655:c3049515-3049387 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT88186 NZ_CP024655:c3049515-3049387 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5