Detailed information of TA system TA088185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961255..961814
• Replicon: chromosome
• Accession: NZ_CP024653
• Organism: Brucella melitensis strain B.melitensis QH61 isolate abortion

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: CT124_RS05035
• Protein ID: WP_002966807.1
• Coordinates: 961569..961814 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: CT124_RS05030
• Protein ID: WP_004683740.1
• Coordinates: 961255..961569 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CT124_RS04980	956960..957163	-	204	WP_004683737.1	hypothetical protein	-
CT124_RS04985	957166..957381	-	216	WP_005968947.1	hypothetical protein	-
CT124_RS04990	957378..957581	-	204	WP_002964088.1	hypothetical protein	-
CT124_RS04995	957631..958335	+	705	WP_005968944.1	hypothetical protein	-
CT124_RS05000	958332..958562	+	231	WP_002964091.1	hypothetical protein	-
CT124_RS05005	958559..958834	+	276	WP_002964092.1	hypothetical protein	-
CT124_RS05010	958857..959333	+	477	WP_002966806.1	hypothetical protein	-
CT124_RS05015	959679..960389	+	711	WP_004683739.1	porin family protein	-
CT124_RS05020	960737..960907	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
CT124_RS17150	960906..961139	+	234	WP_002969999.1	hypothetical protein	-
CT124_RS05030	961255..961569	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
CT124_RS05035	961569..961814	-	246	WP_002966807.1	BrnT family toxin	Toxin
CT124_RS05040	961947..962585	+	639	Protein_923	tRNA-guanine transglycosylase	-
CT124_RS05045	962655..963413	+	759	Protein_924	IS5 family transposase	-
CT124_RS05050	963414..964106	+	693	WP_006135935.1	hypothetical protein	-
CT124_RS05055	964518..965969	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955188..969285	14097
	-	flank	IS/Tn	-	-	962655..963023	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T88185 WP_002966807.1 NZ_CP024653:c961814-961569 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T88185 NZ_CP024653:c961814-961569 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT88185 WP_004683740.1 NZ_CP024653:c961569-961255 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT88185 NZ_CP024653:c961569-961255 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1