Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3127072..3127726 Replicon chromosome
Accession NZ_CP024650
Organism Escherichia coli strain BH100 substr. MG2014

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag CDW44_RS15755 Protein ID WP_000244765.1
Coordinates 3127072..3127479 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CDW44_RS15760 Protein ID WP_000354046.1
Coordinates 3127460..3127726 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDW44_RS15735 3123029..3124762 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
CDW44_RS15740 3124768..3125478 - 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CDW44_RS15745 3125503..3126399 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CDW44_RS15750 3126511..3127032 + 522 WP_001055874.1 flavodoxin FldB -
CDW44_RS15755 3127072..3127479 - 408 WP_000244765.1 protein YgfX Toxin
CDW44_RS15760 3127460..3127726 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CDW44_RS15765 3127969..3128949 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
CDW44_RS15770 3129026..3129685 - 660 WP_000250274.1 hemolysin III family protein -
CDW44_RS15775 3129849..3130160 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CDW44_RS15780 3130159..3131637 + 1479 WP_023152188.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T88169 WP_000244765.1 NZ_CP024650:c3127479-3127072 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T88169 NZ_CP024650:c3127479-3127072 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT88169 WP_000354046.1 NZ_CP024650:c3127726-3127460 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88169 NZ_CP024650:c3127726-3127460 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References