Detailed information of TA system TA088153

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 367865..368700
• Replicon: chromosome
• Accession: NZ_CP024650
• Organism: Escherichia coli strain BH100 substr. MG2014

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: CDW44_RS01815
• Protein ID: WP_000854700.1
• Coordinates: 368323..368700 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: CDW44_RS01810
• Protein ID: WP_001278232.1
• Coordinates: 367865..368233 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDW44_RS01790	365525..366343	+	819	WP_001234709.1	DUF945 domain-containing protein	-
CDW44_RS01795	366435..366920	+	486	WP_000213723.1	antirestriction protein	-
CDW44_RS01800	366936..367412	+	477	WP_001186199.1	RadC family protein	-
CDW44_RS01805	367481..367702	+	222	WP_000692353.1	DUF987 domain-containing protein	-
CDW44_RS01810	367865..368233	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CDW44_RS01815	368323..368700	+	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
CDW44_RS01820	368697..369185	+	489	WP_000761726.1	hypothetical protein	-
CDW44_RS01825	369197..369394	+	198	WP_000839247.1	DUF957 domain-containing protein	-
CDW44_RS01830	369479..370333	+	855	WP_024174313.1	DUF4942 domain-containing protein	-
CDW44_RS01835	370426..371745	-	1320	WP_000144686.1	site-specific integrase	-
CDW44_RS01840	372111..372449	+	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
CDW44_RS01845	372884..373378	-	495	WP_001059463.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vat / yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	342133..417679	75546

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T88153 WP_000854700.1 NZ_CP024650:368323-368700 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T88153 NZ_CP024650:368323-368700 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT88153 WP_001278232.1 NZ_CP024650:367865-368233 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT88153 NZ_CP024650:367865-368233 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A1I2

Antitoxin

• AlphaFold DB: E3PJ72