88147

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 482277..482914 Replicon chromosome
Accession NZ_CP024647
Organism Bacillus sp. Lzh-5

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CS547_RS02470 Protein ID WP_003156187.1
Coordinates 482564..482914 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CS547_RS02465 Protein ID WP_003156188.1
Coordinates 482277..482558 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS547_RS02445 478642..479241 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
CS547_RS02450 479334..479699 + 366 WP_022552632.1 holo-ACP synthase -
CS547_RS02455 479864..480871 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
CS547_RS02460 480988..482157 + 1170 WP_022552633.1 alanine racemase -
CS547_RS02465 482277..482558 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CS547_RS02470 482564..482914 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CS547_RS02475 483032..483853 + 822 WP_003156182.1 STAS domain-containing protein -
CS547_RS02480 483858..484223 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
CS547_RS02485 484226..484627 + 402 WP_003156178.1 anti-sigma regulatory factor -
CS547_RS02490 484639..485646 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
CS547_RS02495 485710..486039 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CS547_RS02500 486036..486518 + 483 WP_007609591.1 anti-sigma B factor RsbW -
CS547_RS02505 486484..487272 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CS547_RS02510 487272..487874 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T88147 WP_003156187.1 NZ_CP024647:482564-482914 [Bacillus sp. Lzh-5]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T88147 NZ_CP024647:482564-482914 [Bacillus sp. Lzh-5]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT88147 WP_003156188.1 NZ_CP024647:482277-482558 [Bacillus sp. Lzh-5]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT88147 NZ_CP024647:482277-482558 [Bacillus sp. Lzh-5]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References