Detailed information of TA system TA088140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 300736..301379
• Replicon: chromosome
• Accession: NZ_CP024646
• Organism: Pseudomonas syringae isolate inb918

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A345RIP1
• Locus tag: CT157_RS01290
• Protein ID: WP_065261145.1
• Coordinates: 300736..300918 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CT157_RS01295
• Protein ID: WP_178081275.1
• Coordinates: 300954..301379 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CT157_RS01270	295816..296820	-	1005	WP_127796604.1	D-cysteine desulfhydrase	-
CT157_RS01275	297011..297937	+	927	WP_127796605.1	serine acetyltransferase	-
CT157_RS01280	298226..300187	+	1962	WP_164872166.1	choline transporter BetT	-
CT157_RS01285	300211..300471	-	261	WP_007952727.1	hypothetical protein	-
CT157_RS01290	300736..300918	+	183	WP_065261145.1	type II toxin-antitoxin system HicA family toxin	Toxin
CT157_RS01295	300954..301379	+	426	WP_178081275.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CT157_RS01300	301402..302439	-	1038	WP_127796607.1	LacI family DNA-binding transcriptional regulator	-
CT157_RS01305	302646..304871	+	2226	WP_127796608.1	TonB-dependent receptor	-
CT157_RS01310	304873..306192	+	1320	WP_127796609.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-135)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6836.96 Da
• Isoelectric Point: 10.5417

>T88140 WP_065261145.1 NZ_CP024646:300736-300918 [Pseudomonas syringae]
MRSREMIRMIEEDGWYLVAVKGSHHQYKHPGKPGRVTIKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T88140 NZ_CP024646:300736-300918 [Pseudomonas syringae]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAGGAGGATGGCTGGTATCTGGTCGCCGTCAAAGGCAGTCATCACCAATA
CAAACATCCGGGCAAGCCAGGGAGGGTGACGATCAAGCATCCGGATTCGGATCTCCCCAAGGGTACGATCAACAGCATCT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15466.29 Da
• Isoelectric Point: 4.2513

>AT88140 WP_178081275.1 NZ_CP024646:300954-301379 [Pseudomonas syringae]
MSEEGLTYMKFPVVLHKDADSDYGVIIPDVPGCFSAGTTVAEAFDNTQEALALHYEGLVADGAPLPRVQDIDAHLENPDY
AGGIWGVVEFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYSSRSGFLAAAALRELSA

Nucleotide

• Length: 426 bp

>AT88140 NZ_CP024646:300954-301379 [Pseudomonas syringae]
ATGTCAGAGGAAGGACTCACTTACATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCAGACTACGGAGTGATTAT
CCCGGATGTACCGGGGTGCTTCTCCGCAGGCACTACGGTAGCCGAAGCTTTTGACAACACTCAGGAGGCACTGGCCTTGC
ACTATGAGGGATTGGTCGCTGATGGCGCGCCGTTACCTCGGGTTCAGGACATTGATGCCCATCTCGAGAATCCGGATTAC
GCCGGCGGCATCTGGGGTGTGGTCGAGTTTGATATCACCCCCTATTTCGGCAAGTCGGTGCGCTTCAACGCTACGCTGCC
GGAGCAGTTGCTGGAACGTATCGATCAGACGGTTCGGCGTGACCAGCGCTACAGTTCGCGCTCCGGATTTTTGGCCGCTG
CTGCCTTGCGCGAGTTGTCGGCATAA

Structures

Toxin

• AlphaFold DB: A0A345RIP1