Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2802872..2803491 Replicon chromosome
Accession NZ_CP024643
Organism Klebsiella michiganensis strain F107

Toxin (Protein)


Gene name Hha Uniprot ID J5X5R4
Locus tag CHQ91_RS15770 Protein ID WP_004848333.1
Coordinates 2803273..2803491 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J4WQ74
Locus tag CHQ91_RS15765 Protein ID WP_009653563.1
Coordinates 2802872..2803246 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHQ91_RS15755 2798031..2799224 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CHQ91_RS15760 2799247..2802393 + 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit -
CHQ91_RS15765 2802872..2803246 + 375 WP_009653563.1 Hha toxicity modulator TomB Antitoxin
CHQ91_RS15770 2803273..2803491 + 219 WP_004848333.1 hemolysin expression modulator Hha Toxin
CHQ91_RS15775 2803653..2804219 + 567 WP_004848331.1 maltose O-acetyltransferase -
CHQ91_RS15780 2804346..2804816 + 471 WP_004848329.1 YlaC family protein -
CHQ91_RS15785 2804791..2806245 - 1455 WP_049077911.1 PLP-dependent aminotransferase family protein -
CHQ91_RS15790 2806347..2807045 + 699 WP_128349260.1 GNAT family N-acetyltransferase -
CHQ91_RS15795 2807042..2807182 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
CHQ91_RS15800 2807182..2807445 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8626.03 Da        Isoelectric Point: 8.9008

>T88133 WP_004848333.1 NZ_CP024643:2803273-2803491 [Klebsiella michiganensis]
MSDKTLTKVDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T88133 NZ_CP024643:2803273-2803491 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAGTAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCGGCTGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14378.14 Da        Isoelectric Point: 4.8989

>AT88133 WP_009653563.1 NZ_CP024643:2802872-2803246 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPVSLSC

Download         Length: 375 bp

>AT88133 NZ_CP024643:2802872-2803246 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGGCATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCGGGCAATCGGTT
ATTCCGCTGTTTTGTCAACGCCAGTCGGGAAAACCCGGTCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R4L0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R4I8

References