Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 473099..473689 Replicon chromosome
Accession NZ_CP024643
Organism Klebsiella michiganensis strain F107

Toxin (Protein)


Gene name pemK Uniprot ID A0A7H4NGB4
Locus tag CHQ91_RS04750 Protein ID WP_064377728.1
Coordinates 473357..473689 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag CHQ91_RS04745 Protein ID WP_004852307.1
Coordinates 473099..473356 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHQ91_RS04720 468266..468874 - 609 WP_161505339.1 glutathione S-transferase family protein -
CHQ91_RS04725 469051..469977 + 927 WP_064377732.1 LysR family transcriptional regulator -
CHQ91_RS04730 470015..471013 - 999 WP_064377731.1 aldo/keto reductase -
CHQ91_RS04735 471114..472028 + 915 WP_064377730.1 LysR family transcriptional regulator -
CHQ91_RS29625 472147..472329 - 183 WP_064377729.1 hypothetical protein -
CHQ91_RS04740 472541..472747 + 207 WP_074177945.1 helix-turn-helix domain-containing protein -
CHQ91_RS04745 473099..473356 + 258 WP_004852307.1 antitoxin Antitoxin
CHQ91_RS04750 473357..473689 + 333 WP_064377728.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CHQ91_RS04760 474012..475469 + 1458 WP_025106072.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
CHQ91_RS04770 476431..477885 - 1455 WP_014229909.1 AMP nucleosidase -
CHQ91_RS04775 478018..478275 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11809.68 Da        Isoelectric Point: 10.5834

>T88124 WP_064377728.1 NZ_CP024643:473357-473689 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T88124 NZ_CP024643:473357-473689 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTAGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGCGGAAACTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAACGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT88124 WP_004852307.1 NZ_CP024643:473099-473356 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT88124 NZ_CP024643:473099-473356 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4NGB4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References