Detailed information of TA system TA088123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 120560..121203
• Replicon: plasmid unnamed2
• Accession: NZ_CP024642
• Organism: Klebsiella michiganensis strain F107

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CHQ91_RS02425
• Protein ID: WP_048295728.1
• Coordinates: 120560..120976 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: CHQ91_RS02430
• Protein ID: WP_001261276.1
• Coordinates: 120973..121203 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ91_RS02410	116141..117163	-	1023	WP_001567660.1	IS110 family transposase	-
CHQ91_RS02415	117303..117611	+	309	WP_001568014.1	hypothetical protein	-
CHQ91_RS02420	118252..120390	+	2139	WP_048295729.1	AAA family ATPase	-
CHQ91_RS02425	120560..120976	-	417	WP_048295728.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHQ91_RS02430	120973..121203	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CHQ91_RS02435	121566..123527	-	1962	WP_048295727.1	tetratricopeptide repeat protein	-
CHQ91_RS02440	124211..125458	-	1248	WP_048295726.1	hypothetical protein	-
CHQ91_RS02445	125606..125884	+	279	WP_048295725.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..144739	144739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15094.58 Da
• Isoelectric Point: 8.5403

>T88123 WP_048295728.1 NZ_CP024642:c120976-120560 [Klebsiella michiganensis]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPALVLEDWVK

Nucleotide

• Length: 417 bp

>T88123 NZ_CP024642:c120976-120560 [Klebsiella michiganensis]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCTGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGCCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT88123 WP_001261276.1 NZ_CP024642:c121203-120973 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT88123 NZ_CP024642:c121203-120973 [Klebsiella michiganensis]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023