Detailed information of TA system TA088121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 193449..193971
• Replicon: plasmid unnamed1
• Accession: NZ_CP024641
• Organism: Klebsiella michiganensis strain F107

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: CHQ91_RS01135
• Protein ID: WP_014839901.1
• Coordinates: 193449..193733 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: CHQ91_RS01140
• Protein ID: WP_014839900.1
• Coordinates: 193723..193971 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ91_RS01100	188705..188914	+	210	WP_125316032.1	hypothetical protein	-
CHQ91_RS01105	188977..189921	+	945	WP_014839903.1	DsbA family protein	-
CHQ91_RS01110	190002..190523	+	522	WP_048336588.1	hypothetical protein	-
CHQ91_RS01115	190520..191122	+	603	WP_014839902.1	hypothetical protein	-
CHQ91_RS01120	191363..192082	+	720	WP_042934502.1	cation:proton antiporter	-
CHQ91_RS01125	192099..192776	-	678	WP_042934501.1	hypothetical protein	-
CHQ91_RS01130	192782..193258	-	477	WP_117044267.1	hypothetical protein	-
CHQ91_RS01135	193449..193733	-	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CHQ91_RS01140	193723..193971	-	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CHQ91_RS01145	194312..195715	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
CHQ91_RS01150	195744..196376	-	633	WP_001567369.1	hypothetical protein	-
CHQ91_RS01155	196920..197528	-	609	WP_024266306.1	DUF2913 family protein	-
CHQ91_RS01160	197665..198063	-	399	WP_014839899.1	H-NS histone family protein	-
CHQ91_RS01165	198437..198793	-	357	WP_017901071.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14	-	1..308586	308586
	-	flank	IS/Tn	-	-	194312..195715	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T88121 WP_014839901.1 NZ_CP024641:c193733-193449 [Klebsiella michiganensis]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T88121 NZ_CP024641:c193733-193449 [Klebsiella michiganensis]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT88121 WP_014839900.1 NZ_CP024641:c193971-193723 [Klebsiella michiganensis]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT88121 NZ_CP024641:c193971-193723 [Klebsiella michiganensis]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5