Detailed information of TA system TA088093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4610481..4611112
• Replicon: chromosome
• Accession: NZ_CP024618
• Organism: Escherichia coli strain SMN152SH1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CR916_RS26470
• Protein ID: WP_032203651.1
• Coordinates: 4610837..4611112 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: CR916_RS26465
• Protein ID: WP_000593555.1
• Coordinates: 4610481..4610840 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR916_RS26440	4606613..4607557	+	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-
CR916_RS26445	4607554..4608387	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
CR916_RS26450	4608387..4609151	+	765	WP_062882693.1	nickel import ATP-binding protein NikD	-
CR916_RS26455	4609148..4609954	+	807	WP_062882694.1	nickel import ATP-binding protein NikE	-
CR916_RS26460	4609960..4610361	+	402	WP_062882695.1	nickel-responsive transcriptional regulator NikR	-
CR916_RS26465	4610481..4610840	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CR916_RS26470	4610837..4611112	-	276	WP_032203651.1	type II toxin-antitoxin system HicA family toxin	Toxin
CR916_RS26475	4611185..4612309	-	1125	WP_001216257.1	ABC transporter permease	-
CR916_RS26480	4612309..4615044	-	2736	WP_000149164.1	ribosome-associated ATPase/putative transporter RbbA	-
CR916_RS26485	4615041..4616108	-	1068	WP_000361467.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10213.97 Da
• Isoelectric Point: 10.7076

>T88093 WP_032203651.1 NZ_CP024618:c4611112-4610837 [Escherichia coli]
MRTKLQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T88093 NZ_CP024618:c4611112-4610837 [Escherichia coli]
ATGAGAACCAAGTTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT88093 WP_000593555.1 NZ_CP024618:c4610840-4610481 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT88093 NZ_CP024618:c4610840-4610481 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6