Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4045333..4045987 Replicon chromosome
Accession NZ_CP024618
Organism Escherichia coli strain SMN152SH1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CR916_RS23285 Protein ID WP_000244777.1
Coordinates 4045333..4045740 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CR916_RS23290 Protein ID WP_000354046.1
Coordinates 4045721..4045987 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR916_RS23265 4041291..4043024 - 1734 WP_024226240.1 single-stranded-DNA-specific exonuclease RecJ -
CR916_RS23270 4043030..4043740 - 711 WP_024226239.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CR916_RS23275 4043764..4044660 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CR916_RS23280 4044772..4045293 + 522 WP_001055874.1 flavodoxin FldB -
CR916_RS23285 4045333..4045740 - 408 WP_000244777.1 toxin CptA Toxin
CR916_RS23290 4045721..4045987 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CR916_RS23300 4046230..4047210 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CR916_RS23305 4047406..4048065 - 660 WP_000250274.1 hemolysin III family protein -
CR916_RS23310 4048229..4048540 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CR916_RS23315 4048585..4050018 + 1434 WP_062882117.1 6-phospho-beta-glucosidase BglA -
CR916_RS23320 4050075..4050818 - 744 WP_062882111.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T88090 WP_000244777.1 NZ_CP024618:c4045740-4045333 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T88090 NZ_CP024618:c4045740-4045333 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT88090 WP_000354046.1 NZ_CP024618:c4045987-4045721 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88090 NZ_CP024618:c4045987-4045721 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References